Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases

By E. Perez, Jianbin Wang, Jeffrey C. Miller, Y. Jouvenot, Kenneth A Kim, O. Liu, Nathaniel S. Wang, Gary K. Lee, V. Bartsevich, Ya-Li Lee and 13 more. Nature Biotechnology, 2008

1,058 citations recorded by the source corpus.

Subfields: Immunology, Molecular Biology, Virology

View Paper: https://www.semanticscholar.org/paper/2edb179f37eee54bd7a5bdb6278df296c0eb36cf

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Citations in the corpus

Reprogramming homing endonuclease specificity through computational design and directed evolution (2013, background): “This process can be used to generate directed modiﬁcations in animal models and shows potential for human gene therapy (2).”
Applications of genome editing technology in the targeted therapy of human diseases: mechanisms, advances and prospects (2020, background): “primary human CD4+ T cells.(138) Approximately 50% of the CCR5 alleles were disrupted with ZFN, which was delivered by the chimeric Ad5/F35 adenoviral vector.”
The discovery of zinc fingers and their applications in gene regulation and genome manipulation. (2010, methodology): “was done by Sangamo in their experiments on establishing HIV resistance in CD4 T cells by genome editing (50).”
CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity (2013, background): “For example, engineered zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) have generated hundreds of animal models for disease studies (1,2), and nuclease-based treatment strategies are currently undergoing clinical trials.”
Cas-analyzer: an online tool for assessing genome editing results using NGS data (2016, methodology): “The induced mutation rates in cells can be estimated in a straightforward manner by using Surveyor nuclease (Perez et al., 2008), the T7 endonuclease I (T7E1) assay (Kim et al., 2009), polyacrylamide gel electrophoresis (Zhu et al., 2014) or droplet digital PCR (Nelson et al., 2016).”
Zinc finger nuclease-mediated CCR5 knockout hematopoietic stem cell transplantation controls HIV-1 in vivo (2010, positive): “Overall, we consider that any adverse effects on cell viability resulting from the nucleofection procedure may be offset by the high levels of CCR5 disruption achieved, as well as the speed and simplicity of the procedure when compared to viral vector systems (19,21).”
HIV: cell binding and entry. (2012, background): “Finally, attempts to recapitulate the ccr5D32 phenotype have been developed with some being brought forward to early-stage clinical development (Perez et al. 2008).”
Genome Engineering With Zinc-Finger Nucleases (2011, methodology): “The ZFNs employed in a current clinical trial have been selected, refined, and tested extensively (Perez et al. 2008; Urnov et al. 2010).”
Genome editing with engineered zinc finger nucleases (2010, background): “Also, the protein and DNA must interact at the correct positions across the entire recognition interface to ensure efficient binding in vivo”