Three decades of nanopore sequencing

By David Deamer, Mark Akeson, D. Branton. Nature Biotechnology, 2016

808 citations recorded by the source corpus.

A long-held goal in sequencing has been to use a voltage-biased nanoscale pore in a membrane to measure the passage of a linear, single-stranded (ss) DNA or RNA molecule through that pore. With the development of enzyme-based methods that ratchet polynucleotides through the nanopore, nucleobase-by-nucleobase, measurements of changes in the current through the pore can now be decoded into a DNA sequence using an algorithm. In this Historical Perspective, we describe the key steps in nanopore strand-sequencing, from its earliest conceptualization more than 25 years ago to its recent commercialization and application.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/2ec34500cf1d4db26f03ea6bdb4a55a969ad8f9f

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Citations in the corpus

Sub-10 nm fabrication: methods and applications (2021, methodology): “(a) Reprinted by permission from Springer Nature Customer Service Centre GmbH: Nature Biotechnology, Nature Biotechnology [41].”
Nanopore sequencing technology, bioinformatics and applications (2021, background): “The concept of nanopore sequencing emerged in the 1980s and was realized through a series of technical advances in both the nanopore and the associated motor protein 1,4–8 . α -Hemolysin, a membrane channel protein from Staphylococcus aureus with an internal diameter of ~1.4nm to ~2.4nm (refs.”
The Third Revolution in Sequencing Technology. (2018, background): “Nanopore Sequencing: ONT The idea of using nanopores in a membrane to sequence single-stranded (ss) DNA or RNA molecules originated at the end of the 1980s [21].”
From squiggle to basepair: computational approaches for improving nanopore sequencing read accuracy (2018, methodology): “The nanopore sequencing concept was first proposed in the 1980s and has been developed and refined over the past three decades (reviewed in [1]).”
SqueezeMeta, A Highly Portable, Fully Automatic Metagenomic Analysis Pipeline (2018, background): “Lately, the development of highly portable sequencers, especially those based on nanopore technologies (Deamer et al., 2016), has facilitated in situ sequencing in scenarios where the need to obtain quick results is paramount, for instance clinical scenarios of disease control or epidemics (Quick et”