Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits.

By Kristina D. Micheva, Stephen J. Smith. Neuron, 2007

806 citations recorded by the source corpus.

Subfields: Biophysics, Structural Biology

View Paper: https://www.semanticscholar.org/paper/2bf96eaf6be72b18a1a16aee98e735dc78345ad7

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Citations in the corpus

Propagation of tau pathology in a model of early Alzheimer’s disease (2012, positive): “=3 at 21 months, 3 at 24 months old) mice and littermate controls (n=5 at 21 months, 3 at 24 months old) were prepared for array tomography as described previously (Koffie et al., 2009; Micheva and Smith, 2007).”
The Angelman Syndrome-associated ubiquitin ligase Ube3A regulates synapse development by ubiquitinating Arc (2010, positive): “To determine whether GluR1 expression at synapses is dysregulated in Ube3A knockout neurons in the context of an intact neuronal circuit, we employed array tomography, a technique in which ultra-thin sections of brain tissue are stained, imaged, and synapses visualized as a 3D reconstruction (Michev”
The classical complement cascade mediates CNS synapse elimination. (2007, methodology): “In order to better visualize C1q’s synaptic localization, we turned to array tomography, a powerful new imaging technique that significantly improves the spatial resolution of closely apposed synaptic proteins over confocal microscopy (Micheva and Smith, 2007).”
Structural and molecular interrogation of intact biological systems (2013, neutral): “(5) Perfuse the animal transcardially, first with 20 ml of ice-cold 1× PBS and then 20 ml of the ice-cold hydrogel solution.”
Microglia Sculpt Postnatal Neural Circuits in an Activity and Complement-Dependent Manner (2012, positive): “Array Tomography Array tomography was performed as previously described with minor modifications (Greer et al., 2010; Margolis et al., 2010; Micheva and Smith, 2007; Ross et al., 2010; Stevens et al., 2007).”
On simplicity and complexity in the brave new world of large-scale neuroscience. (2015, background): “Introduction Experimental neuroscience is entering a golden age marked by the advent of remarkable new methods enabling us to record ever increasing numbers of neurons [1– 5,6 ], and measure brain connectivity at various levels of resolution [7–10,11 ,12–14], sometimes measuring both connectivity an”
Expansion Microscopy (2015, positive): “Although acrylate esters have been used for antigen-preserving embedding for electron microscopy ((12),(13)), ExM represents the first use of an embedded polyelectrolyte gel, used here to expand the specimen.”
Genetic dissection of neural circuits. (2008, neutral): “A clever combination of EM and optical microscopy techniques (e.g., Micheva and Smith, 2007) may ultimately be required to reconstruct wiring diagrams at the synaptic level.”
A Computational Framework for Ultrastructural Mapping of Neural Circuitry (2009, background): “[70] and Micheva and Smith [7]) for ssTEMcompliance to augment our CMP library.”