Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons.

By Ian R. Wickersham, D. Lyon, R. J. Barnard, Takuma Mori, S. Finke, K. Conzelmann, J. Young, Edward M. Callaway. Neuron, 2007

1,181 citations recorded by the source corpus.

Subfields: Cellular and Molecular Neuroscience, Cognitive Neuroscience

View Paper: https://www.semanticscholar.org/paper/2bda4a60ca5d273fb9092aae3f7db5730fd86caf

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In Cognitive Neuroscience

Citations in the corpus

Young dentate granule cells mediate pattern separation, whereas old granule cells facilitate pattern completion. (2012, methodology): “To examine synaptic inputs to young (3-week-old) adult-born GCs, we injected another Moloney virus (Figure 4A, Virus 1A) and an engineered rabies virus (Figure 4B, Virus 2) (Wickersham et al., 2007) into the DGs of activated DG-TeTX mice and control littermates (see Experimental Procedures).”
Transneuronal Circuit Tracing with Neurotropic Viruses (2008, positive): “When neurons expressed TVA and not RG, infection with EnvA-SADdG-GFP (and GFP expression from the rabies genome) was restricted to TVA cells and did not spread from those cells [35 ].”
Whole-brain mapping of direct inputs to midbrain dopamine neurons. (2012, positive): “We used the modified rabies virus SADDG-GFP(EnvA), which has two key modifications that determine the specificity of its initial infection and transsynaptic spread (Wickersham et al., 2007b).”
A Sensorimotor Circuit in Mouse Cortex for Visual Flow Predictions. (2017, neutral): “A24b/M2 Is One of the Main Inputs to V1 and Mainly Targets Layer 2/3 and Layer 6 Excitatory Neurons and PV Interneurons To survey all afferents to V1 and identify potential motor-related input, we used transsynaptic rabies tracing (Wickersham et al., 2007).”
Cortical representations of olfactory input by transsynaptic tracing (2010, neutral): “Cells labelled with both histone–GFP (nGFP) and mCherry (arrowheads in c (1), magnified in c (3)) can be distinguished from cells labelledwithmCherry alone, which are foundnear the injection site (c (1)), in the contralateralmotor cortex (c (2)), in the somatosensory barrel cortex (top right; magnif”
Evoked axonal oxytocin release in the central amygdala attenuates fear response. (2012, positive): “monosynaptically restricted retrograde transsynaptic transmission of PS-Rab (Wickersham et al., 2007).”
Genetic manipulation of genes and cells in the nervous system of the fruit fly (2011, positive): “Trans-neuronal tracers based on lectins and neurotrophic viruses have been used to propose connectivity in vertebrate systems (Horowitz et al., 1999; Wickersham et al., 2007), but none have yet been successfully adapted for use in flies.”
Circuit Architecture of VTA Dopamine Neurons Revealed by Systematic Input–Output Mapping (2015, methodology): “To address these limitations, we utilized rabies virus-based andcell-type-specificmonosynaptic tracing techniques (Wickersham et al., 2007; Callaway and Luo, 2015), as well as a viral-genetic strategy that permits whole-brain mapping of input-output relationships of genetically defined neuronal popu”
Intact-Brain Analyses Reveal Distinct Information Carried by SNc Dopamine Subcircuits (2015, methodology): “…utilizing a GFP-expressing rabies virus (RVdGGFP) that both lacks the glycoprotein needed to spread transsynaptically and is pseudotyped with EnvA (so that it can infect only neurons expressing TVA, an avian receptor protein normally absent in mammalian cells [Wickersham et al., 2007]).”
The excitatory neuronal network of the C2 barrel column in mouse primary somatosensory cortex. (2009, background): “…remarkable technical progress is being made toward largescale, high-resolution analysis of synaptic circuits (Nikolenko et al., 2007; Nagel et al., 2003; Boyden et al., 2005; Arenkiel et al., 2007; Petreanu et al., 2007; Wang et al., 2007; Briggman and Denk, 2006; Wickersham et al., 2007).”