Cortical representations of olfactory input by transsynaptic tracing
By Kazunari Miyamichi, F. Amat, F. Moussavi, Chen Wang, Ian R. Wickersham, Nicholas R. Wall, H. Taniguchi, Bosiljka Tasic, Z. J. Huang, Zhigang He and 3 more. 2010
531 citations recorded by the source corpus.
In the mouse, each class of olfactory receptor neurons expressing a given odorant receptor has convergent axonal projections to two specific glomeruli in the olfactory bulb, thereby creating an odour map. However, it is unclear how this map is represented in the olfactory cortex. Here we combine rabies-virus-dependent retrograde mono-trans-synaptic labelling with genetics to control the location, number and type of ‘starter’ cortical neurons, from which we trace their presynaptic neurons. We find that individual cortical neurons receive input from multiple mitral cells representing broadly distributed glomeruli. Different cortical areas represent the olfactory bulb input differently. For example, the cortical amygdala preferentially receives dorsal olfactory bulb input, whereas the piriform cortex samples the whole olfactory bulb without obvious bias. These differences probably reflect different functions of these cortical areas in mediating innate odour preference or associative memory. The trans-synaptic labelling method described here should be widely applicable to mapping connections throughout the mouse nervous system.
Subfields: Cellular and Molecular Neuroscience, Nutrition and Dietetics, Sensory Systems
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Cellular and Molecular Neuroscience
In Sensory Systems
Citations in the corpus
- Recognition (2003, neutral): “…body (MB)/Kenyon cells (KCs) in insects—where sparse combinatorial codes are translated into associative memories, patterns, and behavioral decisions (de Belle & Heisenberg, 1994; Dubnau et al., 2001; Heisenberg et al., 1985; McGuire et al., 2001; Davison & Ehlers, 2011; Miyamichi et al., 2011).”
- Odor representations in olfactory cortex: distributed rate coding and decorrelated population activity (2012, unknown): “Neurons in the olfactory bulb project broadly to the cortex without apparent topography (Ghosh et al., 2011; Miyamichi et al., 2011; Nagayama et al., 2010; Ojima et al., 1984; Sosulski et al., 2011) and odor stimulation activates widely distributed neurons in the cortex again without apparent…”
- Dendritic spines and distributed circuits. (2011, unknown): “Consistent with this, excitatory axons from the olfactory bulb activate an apparent random assortment of neurons in the olfactory cortex (Miyamichi et al., 2011; Sosulski et al., 2011; Stettler and Axel, 2009).”
- Retrograde monosynaptic tracing reveals the temporal evolution of inputs onto new neurons in the adult dentate gyrus and olfactory bulb (2013, methodology): “By means of single-cell electroporation, adenoassociated virus-mediated transduction or Cre-mediated recombination in transgenic mice, previous strategies have proved the efficient expression of TVA and G in restricted populations of starter cells in vivo (11, 12).”
- Driving opposing behaviors with ensembles of piriform neurons. (2011, background): “Taken together, these observations imply that the piriform cortex does not use spatial order to map olfactory input (Ghosh et al., 2011; Miyamichi et al., 2011; Sosulski et al., 2011; Stettler and Axel, 2009) or behavioral output.”
- Whole-brain mapping of direct inputs to midbrain dopamine neurons. (2012, unknown): “…the Cre/loxP gene expression system (Gong et al., 2007) with rabies-virus-based transsynaptic retrograde tracing (Wickersham et al., 2007b) to comprehensively identify monosynaptic inputs to a genetically defined neural population (Haubensak et al., 2010; Miyamichi et al., 2011; Wall et al., 2010).”