Assessment of Soil Microbial Community Structure by Use of Taxon-Specific Quantitative PCR Assays

By Noah Fierer, J. A. Jackson, R. Vilgalys, R. B. Jackson. Applied and Environmental Microbiology, 2005

1,548 citations recorded by the source corpus.

Subfields: Ecology, Ecology, Evolution, Behavior and Systematics

View Paper: https://www.semanticscholar.org/paper/2b716a2910ea8a9b198497a7d0d946e4c57a2745

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Citations in the corpus

Impact of long-term fertilization practices on the abundance and composition of soil bacterial communities in Northeast China. (2010, background): “Treatments: control (CK), fertilizer for quantitative analysis of bacteria from environmental samples (Suzuki et al., 2000; Fierer et al., 2005).”
The Ecology of Acidobacteria: Moving beyond Genes and Genomes (2016, background): “…phylum resident to a wide range of habitats around the globe (Chow et al., 2002; Kuske et al., 2002; Gremion et al., 2003; Quaiser et al., 2003; Fierer et al., 2005; Stafford et al., 2005; Janssen, 2006; Sanguin et al., 2006; de Carcer et al., 2007; Frontiers in Microbiology |…”
Grass invasion of a hardwood forest is associated with declines in belowground carbon pools (2010, methodology): “We determined these ratios using the qPCR method described by Fierer et al. (2005b).”
A comprehensive survey of soil acidobacterial diversity using pyrosequencing and clone library analyses (2009, methodology): “(1999) have been used to quantify acidobacterial abundances in specific samples (Felske et al., 2000; Fierer et al., 2005; Fierer et al., 2007a), to target acidobacterial genomic DNA (Quaiser et al.”
Phosphorus availability and microbial community in the rhizosphere of intercropped cereal and legume along a P-fertilizer gradient (2016, methodology): “The abundance of total bacteria and fungi as well as three different phyla (Actinobacteria, α-Proteobacteria, Firmicutes) was determined by quantitative PCR by using taxon-specific 16S rDNA primers described by Fierer et al. (2005) or 18S rDNA primers described by Vainio and Hantula (2000).”
Relative importance of soil properties and microbial community for soil functionality: insights from a microbial swap experiment (2016, methodology): “Bacterial 16S and fungal ITS genes were amplified with the Eub 338–Eub 518 and ITS1–5 8S primer sets, respectively, as described in Fierer et al. (2005).”
Toward an ecological classification of soil bacteria. (2007, methodology): “A series of six to 10 static incubations per sample were used to measure the average rate of soil CO2 production over the course of the 50-day incubation period at 208C, using the method described in Fierer et al. (2005b).”
Microbes on mountainsides: Contrasting elevational patterns of bacterial and plant diversity (2008, neutral): “The PCR conditions used were as follows: 3 min at 94°C 25 cycles of 30 s at 94°C, 30 s at 50°C (60), 60 s at 72°C, and a final extension for 5 min at 72°C.”
Using microbial communities and extracellular enzymes to link soil organic matter characteristics to greenhouse gas production in a tidal freshwater wetland (2014, methodology): “Eub338 and Eub517 were used to target the 16S rRNA gene (Fierer et al. 2005).”
Molecular Techniques to Assess Microbial Community Structure, Function, and Dynamics in the Environment (2011, methodology): “Several sets of 16S and 5.8S rRNA gene primers have been designed for rapid Q-PCR based quantification of soil bacterial and fungal microbial communities (Fierer et al. 2005).”
Soil aggregate size mediates the impacts of cropping regimes on soil carbon and microbial communities (2015, methodology): “, Management of Carbon Sequestration in Soil, CRC. Press, Boca Raton, FL (1997), pp. 1-10 View Record in Scopus Ling et al., 2014 N. Ling, Y. Sun, J. Ma, J. Guo, P. Zhu, C. Peng, G. Yu, W. Ran, S. Guo, Q. ShenResponse of the bacterial diversity and soil enzyme activity in particle-size fractions of ”