Single-molecule imaging of EGFR signalling on the surface of living cells

By Y. Sako, Shigeru Minoghchi, T. Yanagida. Nature Cell Biology, 2000

879 citations recorded by the source corpus.

Subfields: Biophysics, Oncology, Radiology, Nuclear Medicine and Imaging

View Paper: https://www.semanticscholar.org/paper/2b2920215d758bee16191b1cc6f931ae01b1e8cf

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biophysics

1. M. Saxton
2. Roger Tsien
3. Robert E. Campbell
4. Mark M. Davis
5. A. Ting
6. A. Douglass
7. A. Citri
8. R. Vale

In Oncology

1. A. Citri
2. A. Douglass
3. R. Vale
4. Y. Yarden
5. N. Hynes
6. R. Roskoski
7. N. Iqbal
8. N. Iqbal

In Radiology, Nuclear Medicine and Imaging

1. A. Douglass
2. A. Citri
3. R. Vale
4. Y. Yarden
5. N. Iqbal
6. N. Iqbal
7. M. Lacouture

Citations in the corpus

• Near-wall hindered Brownian diffusion of nanoparticles examined by three-dimensional ratiometric total internal reflection fluorescence microscopy (3-D R-TIRFM) (2004, methodology): “TIRFM has been a popular technique for both in vitro and in vivo single-molecule detection (Ishijima and Yanagida 2001; Sako et al. 2000).”
• Robust single particle tracking in live cell time-lapse sequences (2008, methodology): “We demonstrate our approach based on two applications that critically depend on tracking robustness and globality: (1) Accurate, comprehensive lifetime analysis of endocytic clathrin-coated pits (CCPs), and (2) single molecule tracking of the macrophage transmembrane receptor CD36, revealing recepto”
• Single-molecule microscopy reveals plasma membrane microdomains created by protein-protein networks that exclude or trap signaling molecules in T cells. (2005, positive): “The development of techniques for imaging single fluorophores in vitro and in living cells (Sako et al., 2000; Nakada et al., 2003) offers another means of probing membrane structure and the dynamics of proteins in the plasma membrane.”
• EGF–ERBB signalling: towards the systems level (2006, background): “Ligand binding to this pre-dimerized state forms a 2:2 ligand to receptor configuratio”
• SINGLE-PARTICLE TRACKING (2009, background): “With membrane-bound species it is possible to see not just a fluorescent label but single-pair FRET, a measurement useful in studying signaling pathways [107,108].”
• Creating new fluorescent probes for cell biology (2002, background): “Examples of single-molecule studies in living cells include investigations of EGF receptor dimerizatio”
• Epidermal growth factor receptor: mechanisms of activation and signalling. (2003, background): “The 2:2 ligand-EGFR complex forms on the cell surface [72].”