A ratchet-like inter-subunit reorganization of the ribosome during translocation

By Joachim Frank, R. Agrawal. Nature, 2000

838 citations recorded by the source corpus.

Subfields: Molecular Biology, Structural Biology

View Paper: https://www.semanticscholar.org/paper/2910aa9db91c6bda2c25a4fdca2397caed71e462

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Citations in the corpus

Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics (2000, background): “It is clear that independent motion of the head (3′ domain) or platform (central domain) observed in low-resolution cryo-EM studie”
Studies on the structure-function relationship of the bacterial ribosome (2004, methodology): “This rotation is not dependent on the presence of tRNAs, it is accompanied by defined movements of the L7/L12 stalk, a rotational movement of the 30S head and a relative opening of the mRNA channel of the 30S subunit (Frank and Agrawal 2000).”
Structure of Functionally Activated Small Ribosomal Subunit at 3.3 Å Resolution (2000, background): “…post translocational ejection of the E site tRNA. nel relate to the base-pairing states of the accuracy switch (Lodmell and Dahlberg, 1997; Gabashvili et al., 1999b), which depends in turn on the activation stateA Latch at the Entrance of the mRNA Channel of the ribosome (Frank and Agrawal, 2000).”
The spliceosome: design principles of a dynamic RNP machine. (2009, background): “This translocation is driven by eEF2 (EF-G in bacteria) GTP hydrolysis (Rodnina et al., 1997) and involves a ratcheting motion of the small subunit with respect to the large subunit (Frank and Agrawal, 2000).”
GTP hydrolysis by IF2 guides progression of the ribosome into elongation (2009, background): “Structural studies have identified two intersubunit conformations, differing by an 5 counterclockwise rotation of the 30S subunit with respect to the 50S subunit (Frank and Agrawal, 2000).”
Initiation factor 2 stabilizes the ribosome in a semirotated conformation (2015, background): “During the elongation phase of protein synthesis, the movement of tRNAs through the ribosome is accompanied by large-scale conformational changes, such as intersubunit rotation (1), the swiveling of the 30S subunit head (2), and the movement of a mobile domain of the large ribosomal subunit, the L1 ”
Studies on the role of helix 69 of 23S rRNA in the factor-dependent stages of translation initiation, elongation, and termination (2011, background): “This movement is known under the name of “ratchet” (Frank and Agrawal 2000) and is triggered by the formation of deacylated tRNA in the P-site (Zavialov and Ehrenberg 2003).”
Accurate determination of local defocus and specimen tilt in electron microscopy. (2003, background): “, 2001), and other particles with lower or no symmetry (Frank and Agrawal, 2000; Grigorieff, 1998; Ranson et al., 2001).”
Stochastic mechano-chemical kinetics of molecular motors: A multidisciplinary enterprise from a physicist’s perspective (2012, background): “, the step (ii) of translocation) are governed by Brownian ratchet mechanisms [1746, 1686, 1687, 1743, 1747].”