Accurate determination of local defocus and specimen tilt in electron microscopy.

By Joseph A. Mindell, N. Grigorieff. Journal of Structural Biology, 2003

1,518 citations recorded by the source corpus.

Subfields: Materials Chemistry, Molecular Biology, Structural Biology

View Paper: https://www.semanticscholar.org/paper/d1ce594f46d1c4debbf17242525979a10f338b20

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In Materials Chemistry

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Citations in the corpus

Tailored design of protein nanoparticle scaffolds for multivalent presentation of viral glycoprotein antigens (2020, methodology): “The parameters of the contrast transfer function (CTF) were estimated using CTFFIND4 (Mindell and Grigorieff, 2003).”
Insights into Head-Tailed Viruses Infecting Extremely Halophilic Archaea (2013, neutral): “The digitized micrographs were binned to a pixel size of 2.26 Å/pixel, and the contrast transfer function (CTF) parameters of the images were evaluated by using CTFFIND3 (45).”
3D architecture of DNA Pol α reveals the functional core of multi-subunit replicative polymerases (2009, positive): “The contrast transfer function of the microscope for each micrograph was estimated using CTFFIND3 (Mindell and Grigorieff, 2003) and corrected using Bsoft (Heymann, 2001); 12 913 side-view images of the complex were manually extracted using XMIPP (Sorzano et al, 2004) and 2D reference-free…”
Complete subunit architecture of the proteasome regulatory particle (2012, positive): “The contrast transfer function (CTF) of each micrograph was estimated concurrently with data collection using the ACE2 and CTFFind program”
De novo protein structure determination from near-atomic resolution cryo-EM maps (2015, methodology): “The CTFFIND3 (Mindell and Grigorieff, 2003) was used to determine the defocus values.”
New tools for automated high-resolution cryo-EM structure determination in RELION-3 (2018, methodology): “Therefore, in practice it would probably be faster to start from defoci provided by local CTF estimation programs, such as CTFTILT ( Mindell and Grigorieff, 2003 ), Gctf ( Zhang, 2016 ) or Warp ( Tegunov and Cramer, 2018 ).”
Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen (2017, methodology): “CTF estimation was then performed using CTFFind3 (64), and candidate MERS S-2P/G4 projection images were identified using a difference-of-Gaussians approach (61).”
Prefusion structure of a human coronavirus spike protein (2016, methodology): “Particles were picked from a subset of the data employing a difference-of-Gaussians approach 33 and aligned using reference-free 2D classification employing iterative MRA/MSA using a binning factor of two 34 .”