Mouse Vα14i natural killer T cells are resistant to cytokine polarization in vivo

By J. Matsuda, L. Gapin, J. Baron, S. Sidobre, Daniel B. Stetson, M. Mohrs, R. Locksley, M. Kronenberg. Proceedings of the National Academy of Sciences of the United States of America, 2003

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Subfields: Immunology

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Recognition of bacterial glycosphingolipids by natural killer T cells (2005, methodology): “Immune assays Stimulation of T-cell hybridomas on CD1d-coated plates and intracellular cytokine staining of tetramer-positive cells were performed as described in published protocol”
The Linkage of Innate to Adaptive Immunity via Maturing Dendritic Cells In Vivo Requires CD40 Ligation in Addition to Antigen Presentation and CD80/86 Costimulation (2004, methodology): “We did not see a contribution of NKT cells in our assays because IL-4 was not detected in four out of four experiments.”
Induction of Regulatory Properties in Dendritic Cells by Vα14 NKT Cells1 (2005, background): “Although it has been shown that V 14 NKT cells did not lose their IFN- production even after in vivo -GalCer treatment (35), we detected significant loss of IFN- in purified V 14 NKT cells isolated from mice that received three -GalCer injections (Fig.”