Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent

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Regulated Water Deficits Improve Phytochemical Concentration in Lettuce (2010, methodology): “Total phenolic concentration of lettuce leaves was analyzed by a modified FolinCiocalteu reagent method (Ainsworth and Gillespie, 2007) as outlined elsewhere (Oh et al., 2009).”
Microalgae-cyanobacteria–based biostimulant effect on salinity tolerance mechanisms, nutrient uptake, and tomato plant growth under salt stress (2021, methodology): “Phenolics were assayed by homogenizing 20 mg leaf biomass in 2 mL 95% (v/v) methanol, according to Ainsworth and Gillespie (2007).”
Leaf Shape Index, Growth, and Phytochemicals in Two Leaf Lettuce Cultivars Grown under Monochromatic Light-emitting Diodes (2012, positive): “상 추의 총 페놀 함량은 Folin-Ciocalteau 방법으로 결정하였다 (Ainsworth and Gillespie, 2007).”
Exogenous application of moringa leaf extract modulates the antioxidant enzyme system to improve wheat performance under saline conditions (2012, positive): “As non-enzymatic antioxidants, total phenolic content (mg g) of fresh leaf samples were determined at 765 nm using gallic acid as reference standard (Ainsworth and Gillespie 2007).”
Seed Priming with Ascorbic Acid Improves Drought Resistance of Wheat (2013, methodology): “For the estimation of total phenolics, leaf samples were extracted with 95 % methanol, and the phenolics were estimated using Folin–Ciocalteu method (Ainsworth and Gillespie 2007).”