Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids

By C. Théry, S. Amigorena, G. Raposo, Aled Clayton. Current Protocols in Cell Biology, 2006

5,322 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/24c26215a145681a5125c9038123e4faae6cf476

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In Cancer Research

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Citations in the corpus

Hypoxic Tumor-Derived Exosomal miR-301a Mediates M2 Macrophage Polarization via PTEN/PI3Kγ to Promote Pancreatic Cancer Metastasis. (2018, methodology): “The expression ofM2markers (CD206, CD163, IL10, TGFb, and arginase-1) increased in macrophages incubated with hypoxic exosomes compared with normoxic exosomes or PBS (Fig.”
Hypoxic enhancement of exosome release by breast cancer cells (2012, methodology): “Non-reducing conditions were used for CD63 immunoblots due to the sensitivity of the antibody epitope to reducing conditions, as described previously [26].”
Cell-Produced α-Synuclein Is Secreted in a Calcium-Dependent Manner by Exosomes and Impacts Neuronal Survival (2010, methodology): “To confirm that the CM of -synuclein-expressing cells contained exosomes, we isolated pure exosomes from the CM using a validated differential ultracentrifugation procedure (Théry et al., 2006).”
Influence of storage condition on exosome recovery (2016, neutral): “Exosomes are formed by inward budding and scission of vesicles from the limiting endosomal membranes and released from the multivesicular body lumen into the extracellular environment during exocytosis [3,4].”
Extracellular Vesicle Heterogeneity: Subpopulations, Isolation Techniques, and Diverse Functions in Cancer Progression (2018, background): “On the basis of enrichment profiles of ACTN4, CCNY, and EPHA2, the presence of two distinct subpopulations of EXOs previously identified by sucrose DG was confirmed (82).”
Extracellular vesicles derived from human bone marrow mesenchymal stem cells promote angiogenesis in a rat myocardial infarction model (2013, methodology): “The phenotypic profile of MSC-EVs was determined by a bead-based flow cytometry with an array of antibodies that are commonly used for MSC identification according to the protocol described previously [20].”
Optimized exosome isolation protocol for cell culture supernatant and human plasma (2015, background): “Isolating exosomes from plasma is further complicated due to viscosity and density issues (5,22), thereby limiting the purity obtained from ultracentrifugation protocols.”
Using exosomes, naturally-equipped nanocarriers, for drug delivery (2015, methodology): “Exosomes are 40–100 nm sized extracellular membrane-derived vesicles actively secreted by most cell types, in particular, cells of the immune system such as dendritic cells [10], macrophages [14], B cells [15], and T cells [16]; as well as mesenchymal stem cells [17], endothelial [18] and epithelial”
Exosomes and microvesicles: extracellular vesicles for genetic information transfer and gene therapy. (2012, methodology): “Sucrose density gradients can be used to derive pure populations (97), but this method is time-consuming with low yield.”