Production of the antimalarial drug precursor artemisinic acid in engineered yeast

By D. Ro, Eric M. Paradise, M. Ouellet, K. Fisher, Karyn L. Newman, J. Ndungu, Kimberly A. Ho, Rachel Eachus, T. Ham, J. Kirby and 5 more. Nature, 2006

2,785 citations recorded by the source corpus.

Subfields: Molecular Biology, Pharmacology, Public Health, Environmental and Occupational Health

View Paper: https://www.semanticscholar.org/paper/1d508a944ab954ecd90ad1a1132b705f5d5a6c19

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James J. Collins

Citations in the corpus

The second wave of synthetic biology: from modules to systems (2009, methodology): “By modifying their isoprenoid system, Keasling and colleagues constructed an artemisinin biosynthetic pathway in yeast, potentially providing an affordable and reliable source of highly potent antimalarial drug”
Complete biosynthesis of opioids in yeast (2015, positive): “In recent years, researchers have engineered yeast to produce a variety of plant-based natural products (6), most notably artemisinic acid, a precursor to the antimalarial drug artemisinin (7).”
Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis in Saccharomyces cerevisiae (2022, background): “In recent years, synthetic biologists have developed a variety of tools using Saccharomyces cerevisiae as a model microbial chassis [9].”
Engineering microbial consortia: a new frontier in synthetic biology. (2008, background): “From the synthesis of an anti-malarial drug [ 12 ] to the study of microbial genetic competency [13], engineered microbes have advanced technology while providing insight into the workings of the cell.”
Abscisic acid (ABA) treatment increases artemisinin content in Artemisia annua by enhancing the expression of genes in artemisinin biosynthetic pathway (2009, background): “Malaria, a global health problem, threatens 300–500 million people and kills more than one million people annually ( Ro et al. 2006 ).”
Identification and microbial production of a terpene-based advanced biofuel (2011, methodology): “Previously, we engineered the mevalonate pathway in both Escherichia coli and Saccharomyces cerevisiae for overproduction of FPP”
Production of amorphadiene in yeast, and its conversion to dihydroartemisinic acid, precursor to the antimalarial agent artemisinin (2012, methodology): “tase), producing >100 mg∕L artemisinic acid (13).”