16S rRNA gene-based analysis of fecal microbiota from preterm infants with and without necrotizing enterocolitis
By Yunwei Wang, Jeanette D. Hoenig, K. Malin, Sanaa Qamar, E. Petrof, Jun Sun, Dionysios A. Antonopoulos, Eugene Chang, E. Claud. The ISME Journal, 2009
604 citations recorded by the source corpus.
Neonatal necrotizing enterocolitis (NEC) is an inflammatory intestinal disorder affecting preterm infants. Intestinal bacteria have an important function; however no causative pathogen has been identified. The purpose of this study was to determine if there are differences in microbial patterns that may be critical to the development of this disease. Fecal samples from 20 preterm infants, 10 with NEC and 10 matched controls (including 4 twin pairs) were obtained from patients in a single site level III neonatal intensive care unit. Bacterial DNA from individual fecal samples was PCR-amplified and subjected to terminal restriction fragment length polymorphism analysis and library sequencing of the 16S rRNA gene to characterize diversity and structure of the enteric microbiota. The distribution of samples from NEC patients distinctly clustered separately from controls. Intestinal bacterial colonization in all preterm infants was notable for low diversity. Patients with NEC had even less diversity, an increase in abundance of Gammaproteobacteria, a decrease in other bacteria species, and had received a higher mean number of previous days of antibiotics. Our results suggest that NEC is associated with severe lack of microbiota diversity that may accentuate the impact of single dominant microorganisms favored by empiric and widespread use of antibiotics.
Subfields: Molecular Biology, Nutrition and Dietetics, Surgery
View Paper: https://www.semanticscholar.org/paper/19f01d415146e6ff4c9ee711cda5b90b7fefb601
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Citations in the corpus
- The Intestinal Microbiome in Early Life: Health and Disease (2014, result): “Further, culture-independent case–control comparisons sampled the microbiota within a week of diagnosis and found an outgrowth of Proteobacteria in NEC compared to controls (119, 120).”
- Prolonged Initial Empirical Antibiotic Treatment is Associated with Adverse Outcomes in Premature Infants (2011, background): “The lack of bacterial species diversity and abundance of Proteobacteria species associated with widespread use of antibiotics may predispose to inflammatory stimulation that may help explain the susceptibility of premature newborns to LOS and NEC (13, 26).”
- Intestinal microbiota in human health and disease: the impact of probiotics (2011, methodology): “In children who develop an allergic disease later in life, a reduced diversity of faecal microbiota was already observed at 1 week of age (Wang et al. 2009; Niers et al., personal communication).”
- Effects of diurnal variation of gut microbes and high fat feeding on host circadian clock function and metabolism (2015, methodology): “DNA extraction and 16S rRNA amplicon sequencing analysis Extraction of bacterial DNA from fecal and cecal contents was performed as previously described (Wang et al., 2009).”
- The composition of the gut microbiota throughout life, with an emphasis on early life (2015, background): “NEC has recently been linked to the microbiota, where high levels of Proteobacteria are predictive of the disease (55).”