Evidence that ternary complex (eIF2-GTP-tRNA(i)(Met))-deficient preinitiation complexes are core constituents of mammalian stress granules.

By N. Kedersha, Samantha Chen, Natalie Gilks, Wei Li, Ira Miller, J. Stahl, Paul Anderson. Molecular Biology of the Cell, 2002

634 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/19453c62e5412797887bf2c6e559c7a45af6d421

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. R. Parker
2. Paul Anderson
3. N. Kedersha
4. S. Alberti
5. C. Brangwynne
6. Saumya Jain
7. Rohit V. Pappu
8. Ujwal Sheth

Citations in the corpus

• Mammalian stress granules and processing bodies. (2007, background): “These nonpolysomal transcripts are then actively organized into stress granules, a process mediated by a number of RNA-binding proteins (Kedersha et al., 2002).”
• RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation (2020, background): “SGs have been proposed to be sites of translation repression or stabilization of mRNAs and to have a pro-survival function during stress (Kedersha et al., 2002; Kimball et al., 2003; Panas et al., 2016; Protter and Parker, 2016).”
• P-Body Purification Reveals the Condensation of Repressed mRNA Regulons. (2017, background): “Stalled mRNAs that are bound to the small ribosomal subunit and primed to reenter translation are another hallmark of SGs (Kedersha et al., 2002).”
• G3BP–Caprin1–USP10 complexes mediate stress granule condensation and associate with 40S subunits (2016, neutral): “This model is consistent with photobleaching data indicating that G3BP, PABP, TIA-1, and TIAR are only fleetingly present in SGs (Kedersha et al., 2002, 2005; Bley et al., 2015) and with morphologic EM data showing that SGs have heterogeneous substructures (Souquere et al., 2009).”
• Stress granule assembly is mediated by prion-like aggregation of TIA-1. (2004, background): “Cytoplasmic aggregates are rimmed by a corona of particles that resemble ribosomes or ribosomal subunits (Figure 3D, arrowheads); this result is striking as small ribosomal subunits are among the core components of SGs (Anderson and Kedersha, 2002a; Kedersha et al., 2002).”
• Processing bodies require RNA for assembly and contain nontranslating mRNAs. (2005, background): “Stress granules contain translation initiation factors, small ribosomal subunits, and the mRNA-binding proteins TIA and TIA-R (Kedersha et al. 2002), whereas P-bodies contain mRNA decay factors (Ingelfinger et al. 2002; Lykke-Andersen 2002; van Dijk et al. 2002; Cougot et al. 2004).”
• Competing protein-RNA interaction networks control multiphase intracellular organization (2020, background): “…in mammalian cells upon translational arrest and subsequent polysome disassembly, which releases exposed RNA into the cytoplasm (‘‘RNA influx’’) (Boeynaems et al., 2017; Kedersha et al., 1999, 2002, 2016; Kroschwald et al., 2015; Molliex et al., 2015; Wheeler et al., 2016; Wippich et al., 2013).”
• Cytoplasmic foci are sites of mRNA decay in human cells (2004, background): “, 2000), but accumulated in cytoplasmic stress granules after arsenite treatment as described previously (Fig. 3 B, middle; Kedersha et al., 2002).”
• Stress granules: the Tao of RNA triage. (2008, background): “This class includes mRNA transcripts, eIF3, eIF4F (comprising eIF4E, eIF4A and eIF4G), eIF4B, small ribosomal subunits and PABP-1 [3, 32 ].”
• Stress granules and processing bodies are dynamically linked sites of mRNP remodeling (2005, methodology): “We used DU145 cells, which had been previously used to analyze SG components (Kedersha et al., 2002), and induced SGs by the transient transfection of GFP-G3BP, an SG component whose expression induces the assembly of very large SGs readily amenable to microscopic analysis (Tourriere et al., 2003).”
• Eukaryotic stress granules: the ins and outs of translation. (2009, background): “…stress granule components (Table S2), which, combined with interactions with other stress granule Molecular Cell 36, December 24, 2009 ª2009 Elsevier Inc. 937 factors, suggests a possible scaffolding role in stress granule assembly (Eisinger-Mathason et al., 2008; Kedersha et al., 2005).”
• The stress granule transcriptome reveals principles of mRNA accumulation in stress granules (2017, positive): “…the RNA composition of SGs. SGs are known to contain non-translating mRNA from early reports showing that SGs contain PABP, stain positive for poly(A) mRNA, lack large ribosomal subunits, and are sensitive to drugs that alter the translation (Kedersha et al., 2000, 2002, 1999; McEwen et al., 2005).”