A Resource of Cre Driver Lines for Genetic Targeting of GABAergic Neurons in Cerebral Cortex

By H. Taniguchi, Miao He, Priscilla Wu, Sangyong Kim, Raehum Paik, Ken Sugino, Duda Kvitsani, Yu Fu, Jiangteng Lu, Ying Lin and 5 more. 2011

1,821 citations recorded by the source corpus.

Summary A key obstacle to understanding neural circuits in the cerebral cortex is that of unraveling the diversity of GABAergic interneurons. This diversity poses general questions for neural circuit analysis: how are these interneuron cell types generated and assembled into stereotyped local circuits and how do they differentially contribute to circuit operations that underlie cortical functions ranging from perception to cognition? Using genetic engineering in mice, we have generated and characterized ~20 Cre and inducible CreER knockin driver lines that reliably target major classes and lineages of GABAergic neurons. More select populations are captured by intersection of Cre and Flp drivers. Genetic targeting allows reliable identification, monitoring, and manipulation of cortical GABAergic neurons, thereby enabling a systematic and comprehensive analysis from cell fate specification, migration, and connectivity, to their functions in network dynamics and behavior. As such, this approach will accelerate the study of GABAergic circuits throughout the mammalian brain.

Subfields: Cellular and Molecular Neuroscience, Cognitive Neuroscience, Plant Science

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Citations in the corpus

Optogenetic investigation of neural circuits underlying brain disease in animal models (2012, background): “and transgenic rodent lines that increase the range of available specific cellular target”
A novel inhibitory nucleo-cortical circuit controls cerebellar Golgi cell activity (2015, methodology): “Experiments were performed on adult mice (p > 30 days; both males and females) of two mouse lines: the GAD-ires-cre (Taniguchi et al., 2011) and the GlyT2-cre (Husson et al., 2014).”
COMPREHENSIVE CLASSIFICATION OF RETINAL BIPOLAR NEURONS BY SINGLE-CELL TRANSCRIPTOMICS (2016, positive): “1(cre)Zjh mice were used for AAV-stop-GFP infections at P0 (gift of Dr. David Ginty) (Taniguchi et al., 2011).”
Pupil fluctuations track fast switching of cortical states during quiet wakefulness (2014, positive): “We found that our SOM+ Cre driver line (SST-IRES-Cre; Taniguchi et al., 2011) labeled a population of neurons that could be grouped into two distinct classes, which we call Type I and Type II.”
Principles of connectivity among morphologically defined cell types in adult neocortex (2015, positive): “In addition, it has been increasingly recognized that the SOM-Cre driver line (SOM-IRESCre) (31) labels a population of neurons that can be grouped into distinct types both functionally (19, 32) andmorphologically (fig.”
Inhibition of Inhibition in Visual Cortex: The Logic of Connections Between Molecularly Distinct Interneurons (2013, neutral): “Transgenic mice used were: GAD67-GFP (all subpopulations of interneurons)[51], GIN (Sst cells)[52], G42 (Pvalb cells)[53], B13 (Pvalb cells)[39], HTR3a-GFP (Gensat#DH30) (VIP cells and others including layer 1) [25,43], ROSA-tdTomato[54], Pvalb-Cre[20], Sst-Cre[21], and VIP-Cre[21].”
The role of nitric oxide in pre-synaptic plasticity and homeostasis (2013, methodology): “…using targeted knockin of cre-recombinase into the NOS1 gene and subsequent crosses to GFP reporter lines clearly show two populations of NOS1+ GABAergic cells, one of neurogliaform morphology (type II) and the other characterized by long range axonal projections (type I) (Taniguchi et al., 2011).”