Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides.
By A. Knappik, L. Ge, A. Honegger, P. Pack, M. Fischer, G. Wellnhofer, A. Hoess, Joachim Wölle, A. Plückthun, B. Virnekäs. Journal of Molecular Biology, 2000
751 citations recorded by the source corpus.
Subfields: Immunology, Molecular Biology, Radiology, Nuclear Medicine and Imaging
View Paper: https://www.semanticscholar.org/paper/1746d89ba5de9f240665f85d78e686c8ff069de2
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Immunology
In Molecular Biology
In Radiology, Nuclear Medicine and Imaging
Citations in the corpus
- scFv Antibody: Principles and Clinical Application (2012, positive): “In the majority of this applications, selection has been carried out by incubating phage on antigen immobilized onto a solid support, such as Immunotubes [90, 97], microtiter plate wells [98], BIAcore sensor chips [97], or columns [98].”
- Design of novel FN3 domains with high stability by a consensus sequence approach. (2012, methodology): “This approach has been used to increase the stability of antibodies (Steipe et al., 1994; Ohage and Steipe, 1999; Wirtz and Steipe, 1999; Knappik et al., 2000; Visintin et al., 2002; Demarest et al., 2004), SH3 domains (Maxwell and Davidson, 1998), enzymes (Lehmann et al., 2000a,b), DNA-binding…”
- Engineering novel binding proteins from nonimmunoglobulin domains (2005, background): “, an engineering strategy that had already proven useful in antibody engineering approache”
- Cognizance of Molecular Methods for the Generation of Mutagenic Phage Display Antibody Libraries for Affinity Maturation (2019, background): “The large diversity in a synthetic library is afforded by predefined framework designs and the degree of diversification of the CDR [99,100].”