scFv Antibody: Principles and Clinical Application
By Zuhaida Asra Ahmad, S. Yeap, Abdul Manaf Ali, W. Ho, N. Alitheen, M. Hamid. Clinical and Developmental Immunology, 2012
762 citations recorded by the source corpus.
To date, generation of single-chain fragment variable (scFv) has become an established technique used to produce a completely functional antigen-binding fragment in bacterial systems. The advances in antibody engineering have now facilitated a more efficient and generally applicable method to produce Fv fragments. Basically, scFv antibodies produced from phage display can be genetically fused to the marker proteins, such as fluorescent proteins or alkaline phosphatase. These bifunctional proteins having both antigen-binding capacity and marker activity can be obtained from transformed bacteria and used for one-step immunodetection of biological agents. Alternatively, antibody fragments could also be applied in the construction of immunotoxins, therapeutic gene delivery, and anticancer intrabodies for therapeutic purposes. This paper provides an overview of the current studies on the principle, generation, and application of scFv. The potential of scFv in breast cancer research is also discussed in this paper.
Subfields: Ecology, Molecular Biology, Radiology, Nuclear Medicine and Imaging
View Paper: https://www.semanticscholar.org/paper/326316278b1b3a4838e04bfece69bc8e5053e935
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In Molecular Biology
In Radiology, Nuclear Medicine and Imaging
Citations in the corpus
- Cognizance of Molecular Methods for the Generation of Mutagenic Phage Display Antibody Libraries for Affinity Maturation (2019, background): “The scFv is the most common format used for phage display due to the size and availability of both the heavy and light variable chains (VH/VL) for a collective binding effect [22].”
- The making of bispecific antibodies (2017, background): “domains representing the minimal antigen-binding site of an antibody.(34) Due to the single-chain configuration, bispecific”