Selenomethionyl proteins produced for analysis by multiwavelength anomalous diffraction (MAD): a vehicle for direct determination of three‐dimensional structure.
By W. Hendrickson, J. Horton, D. LeMaster. 1990
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Citations in the corpus
- AlphaFold2 and its applications in the fields of biology and medicine (2023, background): “66,67 Secondly, these predicted structures may also be helpful for structure determination of large protein assemblies by cryo-EM, which usually needs structures of the component proteins or their domains as a starting point for fi tting to the cryo-EM densities.”
- Gelatinase-mediated migration and invasion of cancer cells. (2005, methodology): “This has significantly helped in solving X-ray structures by the multiwavelenght anomalous diffraction method (Budisa et al., 1995; Hendrickson et al., 1990).”
- Crystal Structure of the SPOC Domain of the Arabidopsis Flowering Regulator FPA (2016, methodology): “coli B834(DE3) strain using LeMaster media [27] and purified with the same protocol as the native protein.”
- Protein production by auto-induction in high density shaking cultures. (2005, methodology): “Auto-induction for labeling proteins with SeMet for crystallography—Labeling proteins with SeMet is a standard and useful way to obtain phases for structure determination by X-ray crystallography [33].”
- Insights from soft X-rays: the chlorine and sulfur sub-structures of a CK2α/DRB complex (2008, methodology): “The traditional anomalous dispersion approach (MAD [2]) required the artificial derivatization of the protein by soaking the crystal in”
- Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra α-helical domain (2002, methodology): “The presence of 10 methionine residues in the TGEV Mpro molecule suggested that selenomethionine-based multi- wavelength anomalous dispersion (MAD; Hendrickson et al., 1990) could be used to solve the phase problem.”
- Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis. (2005, methodology): “tion and the multiwavelength anomalous diffraction method (Hendrickson et al., 1990).”