pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation

By R. Hellens, E. Edwards, Nicola Leyland, S. Bean, P. Mullineaux. Plant Molecular Biology, 2000

1,668 citations recorded by the source corpus.

Subfields: Biotechnology, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/14bcdf24b7b4b2c501a0c9ec90bda7cdd1ce32aa

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Citations in the corpus

A CRISPR/Cas9 toolkit for multiplex genome editing in plants (2014, methodology): “The pGreen binary vectors were constructed based on a previously reported strategy [41].”
NAC Transcription Factors, NST1 and NST3, Are Key Regulators of the Formation of Secondary Walls in Woody Tissues of Arabidopsis[W][OA] (2007, background): “These genes and 35S:NST3 were constructed from modified vectors derived from pGreenII0029 (Hellens et al., 2000) and p35SSRDXG (Mitsuda et al., 2006).”
Rapid, combinatorial analysis of membrane compartments in intact plants with a multicolor marker set. (2009, methodology): “Construction of pNIGEL host vectors pNIGEL vectors are based on the pGREENII vector series (Hellens et al., 2000; http://www.pgreen.ac.uk).”
Targets of the WRKY53 transcription factor and its role during leaf senescence in Arabidopsis (2004, methodology): “…cDNA fragment that contained the full coding sequence and the 3¢-untranslated region of AtWRKY53 or part of WRKY53 cDNA (position nt 580–880) in sense and antisense orientation separated by an intron into the PRT-W cassette and into pGreen 0229 binary vector, respectively (Hellens et al., 2000).”
Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants (2005, methodology): “The PCR products were digested with appropriate restriction enzymes and these two digested PCR products were used in a 3-way ligation to HindIII-BamHI cut pNOS-7 [24] to produce the NOShpLUC cassette.”
Antagonistic Interaction between Abscisic Acid and Jasmonate-Ethylene Signaling Pathways Modulates Defense Gene Expression and Disease Resistance in Arabidopsis (2004, methodology): “…vector (Hellens et al., 2000) and ligation of the band corresponding to the double 35S promoter/terminator cassette into the pGreenII vector (Hellens et al., 2000) previously linearized by digestion with HindIII, blunt ended using Klenow enzyme, and dephosphorylated using shrimp alkaline…”
Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis (2009, methodology): “In this study, a series of vector plasmids, such as pGBKT7 and pGADT7 (Clontech), pGEX6P (GE Healthcare), and the pGreen series (30), were converted to Gateway destination vectors.”
Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10 (2007, methodology): “…(MP90) transformed with the reporter cassette with 450 ll each of two other Agrobacterium cultures transformed with cassettes containing a MYB TF gene fused to the 35S promoter and a bHLH TF gene in either pART27 (Gleave, 1992) or pGreenII 62- SK binary vectors (Figure S4) (Hellens et al., 2000).”
The Balance between the MIR164A and CUC2 Genes Controls Leaf Margin Serration in Arabidopsis[W] (2006, methodology): “The various constructs used to complement the mir164a-4 mutant were generated by amplifying several fragments of the MIR164A gene by PCR and reconstructing the gene in the pGreenII0229 binary vector (Hellens et al., 2000).”
SCARECROW is involved in positioning the stem cell niche in the Arabidopsis root meristem. (2003, neutral): “promoter in the 35S cassette (Hellens et al. 2000).”