Blue silver: A very sensitive colloidal Coomassie G‐250 staining for proteome analysis

By G. Candiano, M. Bruschi, L. Musante, Laura Santucci, G. Ghiggeri, B. Carnemolla, P. Orecchia, L. Zardi, P. Righetti. Electrophoresis, 2004

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A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC) (2006, methodology): “Add SDS loading buffer to the tube of beads (add enough SDS loading buffer to include the volume of beads), run a 1D SDS–PAGE gel and stain with an MS-compatible protein dye like colloidal Coomassie stai”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, positive): “Presence and purity of BdCAD5 and BdCAD3 was analysed by SDS-PAGE using PageRuler Prestained Protein ladder (Fermentas, CA, USA) and bands were visualized by Coomassie Brilliant Blue G-250 (Candiano et al., 2004).”
Proteome profile and functional classification of proteins in Arabidopsis thaliana (Landsberg erecta) mature pollen (2006, methodology): “Gels were fixed in 50% ethanol with 10% orthophosphoric acid over-night, washed with water (3 · 20 min), and stained in a Colloidal Coomassie Blue G-250 (CCB) solution (0.12% CB, 10% ammonium sulfate, 10% orthophosphoric acid, 20% methanol; Candiano et al. 2004) for 2 days.”
Molecular mechanism of anaerobic ammonium oxidation (2011, methodology): “Gels were stained with colloidal Coomassie blue as described elsewher”