Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins.

By T. Nagai, Shu-ichi Yamada, T. Tominaga, M. Ichikawa, A. Miyawaki. Proceedings of the National Academy of Sciences of the United States of America, 2004

1,068 citations recorded by the source corpus.

Subfields: Biophysics, Cellular and Molecular Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/102c9b4773830c700e6dabb4af166f7a5b669b9b

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Citations in the corpus

Fluorescent protein FRET: the good, the bad and the ugly. (2007, background): “For example, such sensors have been built for assays of intracellular Ca2+ [4, 7 ], cAMP activity [8,9]”
A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging (2016, methodology): “For example, it would be possible to image the primary somatosensory cortex, the primary motor cortex and parts of secondary motor cortex nearly simultaneously (Figure 1A).”
Development of an optimized backbone of FRET biosensors for kinases and GTPases (2011, methodology): “This new backbone enabled us to improve the previously reported FRET biosensors of PKA, ERK, JNK, EGFR/ Abl, Ras, and Rac1 and to develop new FRET biosensors for RSK, S6K, Akt, and PKC Ser/Thr kinases in a short period of time.”
Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators (2009, methodology): “It has been demonstrated that by altering the relative orientations between donor and acceptor fluorescent proteins of a cameleon calcium indicator using a circularly permuted fluorescent protein, dynamic range of FRET signal can be efficiently enhanced (18).”
Aβ plaques lead to aberrant regulation of calcium homeostasis in vivo resulting in structural and functional disruption of neuronal networks (2008, positive): “7) (Nagai et al., 2004); our measured values were used for all subsequent [Ca]i calibrations.”
Optimization of a GCaMP calcium indicator for neural activity imaging (2012, background): “6 (Nagai et al., 2004), and the high-affinity YC-Nano (Horikawa et al.”
Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance (2015, positive): “Ratiometric imaging using FRET-based calcium indicators, such as yellow cameleon (YC) indicators (Horikawa et al., 2010; Nagai et al., 2004; Yamada et al., 2011), is another approach that has the advantage of reduced sensitivity to motion artifacts and essentially permits quantiﬁcation of calcium…”
Fluorescent proteins and their applications in imaging living cells and tissues. (2010, background): “The universality and relative simplicity of such a design have led to the development of numerous FRETbased sensors of this type, including GES for monitoring changes in the concentration of Ca(2) (130, 153, 262, 263, 291, 292, 295, 304, 307, 327, 434), cyclic nucleotides (174, 317, 318, 375, 445, 5”