Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators
By H. Imamura, K. P. Huynh Nhat, H. Togawa, Kenta Saito, R. Iino, Y. Kato-Yamada, T. Nagai, H. Noji. Proceedings of the National Academy of Sciences of the United States of America, 2009
1,039 citations recorded by the source corpus.
Subfields: Biophysics, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/909f3060d881aa3f4ae9ae452bf5b4a629001cd9
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In Molecular Biology
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Citations in the corpus
- Activity-driven local ATP synthesis is required for synaptic function (2014, neutral): “A Quantitative Reporter of Presynaptic ATP Previous optical reporters of ATP have suffered from a number of drawbacks for attacking this problem, including nonlinearity (Imamura et al., 2009; Saito et al., 2012), saturation at expected physiological ATP levels (Berg et al.”
- Consensus guidelines for the detection of immunogenic cell death (2014, background): “Alternatively, intracellular ATP can be monitored in living cells by a fluorescence resonance energy transfer (FRET)-based assay involving a yellow fluorescent protein-cyan fluorescent protein (YFP-CFP) fusion containing a domain that changes its conformation upon ATP binding, hence shifting the spe”
- Overcoming intrinsic multi-drug resistance in melanoma by blocking the mitochondrial respiratory chain of slow-cycling JARID1Bhigh cells (2013, methodology): “Mitochondrial ATP levels were determined using the mitochondrial ATP sensor protein ATEAM as reported (Imamura et al., 2009) and described in the Supplemental Information.”
- Persister formation in Staphylococcus aureus is associated with ATP depletion (2016, neutral): “To establish direct causality, it would be interesting to perform single cell detection of ATP in persisters more directly, such as with a FRET-based sensor 37 , once it is adapted to S. aureus.”