Anti-Inflammatory Activity of Human IgG4 Antibodies by Dynamic Fab Arm Exchange

By M. van der Neut Kolfschoten, J. Schuurman, M. Losen, W. Bleeker, P. Martinez-Martinez, Ellen Vermeulen, Tamara H. den Bleker, L. Wiegman, T. Vink, L. Aarden and 4 more. Science, 2007

978 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology, Radiology, Nuclear Medicine and Imaging

View Paper: https://www.semanticscholar.org/paper/0bbd09b354e9c938b2072fc315ab196622fe6dbf

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In Molecular Biology

1. Dennis R. Goulet
2. M. Chiu
3. Gary Gilliland
4. A. Teplyakov

In Radiology, Nuclear Medicine and Imaging

1. Dennis R. Goulet
2. M. Chiu
3. Gary Gilliland
4. A. Teplyakov

Citations in the corpus

• Tumor-associated and microbial proteases compromise host IgG effector functions by a single cleavage proximal to the hinge (2009, background): “, HPLC, SEC), the masses of intact IgG and the single-cleaved intermediate were indistinguishable because of the core hinge disulfide bridging between Fc and Fab arms and the strong noncovalent associations of the two paired Fc chains in the CH3 regions (30, 31).”
• IgG Subclasses and Allotypes: From Structure to Effector Functions (2014, background): “IgG4 Fab ARM EXCHANGE In vivo, half-molecules of IgG4 recombine randomly with other half-molecules of IgG4, combining specificities of two IgG4 molecules, effectively resulting in monovalent-bispecific antibodies (Figure 2C) (75,76),and is controlled by redox conditions (74,77).”
• Antibody Structure and Function: The Basis for Engineering Therapeutics (2019, background): “Interestingly, the structural oddities of human IgG4, including a labile hinge enabled by S228 (proline in other subclasses) and weaker inter-CH3 interactions caused by R409 (lysine in other subclasses), allow it to undergo a process called Fab-arm exchange in vivo to create bispecific IgG4 antibodi”
• The making of bispecific antibodies (2017, background): “This process was attributed to IgG4 core hinge sequences in conjugation with residues in the CH3 domain.(174) This natural”