Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

By S. Cho, Sojung Kim, Jong Min Kim, Jin-Soo Kim. Nature Biotechnology, 2013

1,899 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/0bb56c4dc7615c490780af5654c3af9aa3fed803

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In Molecular Biology

Citations in the corpus

One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas mediated genome engineering (2013, background): “Because the binding of Cas9 is guided by the simple base-pair complementarities between the engineered single guide RNA (sgRNA) and a target genomic DNA sequence, it is possible to direct Cas9 to any genomic locus, by providing the engineered sgRNA (Cho et al., 2013; Cong et al., 2013; Gilbert et al”
Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair (2017, methodology): “The experimental groups were: (1) negative control, consisting of mdx mice without injection (n = 6), (2) control (scrambled CRISPR–Gold), consisting of mice injected with CRISPR–Gold containing scrambled gRNA (n = 11), (3) CRISPR–Gold, consisting of mice injected with CRISPR–Gold containing RNP and”
RNA-guided genome editing in plants using a CRISPR-Cas system. (2013, background): “The CRISPR/ Cas system has been demonstrated for genome editing in human (Cho et al., 2013; Cong et al., 2013; Jinek et al., 2013; Mali et al., 2013b), mice (Shen et al.”
CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites (2014, background): “The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 nuclease in complex with a guide RNA (gRNA), that complementarily binds to a 20 nt targeted sequence.”
Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. (2013, background): “While our paper was under review, several very recent studies reported the use of wild-type Cas9/CRISPR for targeted genome editing in diverse organisms (Mali et al., 2013; Cong et al., 2013; Jinek et al., 2013; Jiang et al., 2013; Hwang et al., 2013; Cho et al., 2013).”
CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes (2015, background): “…containing the tri-nucleotide protospacer adjacent motif (PAM) and complementary to the gRNA, and can be programmed to recognize virtually any genes through the manipulation of gRNA sequences (Cho et al., 2013; Cong et al., 2013; Jinek et al., 2012; Jinek et al., 2013; Mali et al., 2013c).”
A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia (2016, background): “Cell Rep This is an open access article und transforming the landscape of genetic research in many organisms (Cho et al., 2013; Cong et al., 2013; Jinek et al., 2012; Mali et al., 2013).”
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes (2013, positive): “Recently, several groups have shown that a modified type II CRISPR (Clustered Regularly Interspaced Palindromic Repeats) system can be targeted to DNA using RNA, enabling genetic editing of any region of the genome in a variety of organisms (Cho et al., 2013; Cong et al., 2013; Dicarlo et al., 2013;”
Discovery and functional characterization of diverse Class 2 CRISPR-Cas systems (2015, background): “These systems have recently attracted much attention due to their unique, “Lamarckian” mode of action that retains “memories” from past infections and provides specific resistance to these infections via an RNA-guided process that has been harnessed to create powerful genome editing tools (Cong et a”