Multipotent mesenchymal stem cells from adult human synovial membrane.

By C. Bari, F. Dell’Accio, P. Tylzanowski, Frank P. Luyten. Arthritis & Rheumatism, 2001

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OBJECTIVE To characterize mesenchymal stem cells (MSCs) from human synovial membrane (SM). METHODS Cell populations were enzymatically released from the SM obtained from knee joints of adult human donors and were expanded in monolayer with serial passages at confluence. Cell clones were obtained by limiting dilution. At different passages, SM-derived cells were subjected to in vitro assays to investigate their multilineage potential. Upon treatments, phenotypes of cell cultures were analyzed by histo- and immunohistochemistry and by semiquantitative reverse transcription-polymerase chain reaction for the expression of lineage-retated marker genes. RESULTS SM-derived cells could be expanded extensively in monolayer, with limited senescence. Under appropriate culture conditions, SM-derived cells were induced to differentiate to the chondrocyte, osteocyte, and adipocyte lineages. Sporadic myogenesis was also observed. Five independent cell clones displayed multilineage potential. Interestingly, only 1 clone was myogenic. Donor age, cell passaging, and cryopreservation did not affect the multilineage potential of SM-derived cells. In contrast, normal dermal fibroblasts under the same culture conditions did not display this potential. CONCLUSION Our study demonstrates that human multipotent MSCs can be isolated from the SM of knee joints. These cells have the ability to proliferate extensively in culture, and they maintain their multilineage differentiation potential in vitro, establishing their progenitor cell nature. SM-derived MSCs may play a role in the regenerative response during arthritic diseases and are promising candidates for developing novel cell-based therapeutic approaches for postnatal skeletal tissue repair.

Subfields: Genetics, Molecular Biology, Rheumatology

View Paper: https://www.semanticscholar.org/paper/094b5330bb0c493ac73f3940913d80a56283e1fc

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Citations in the corpus

Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle (2007, background): “Furthermore, De Bari et al. (2001b) have replated synovium-derived MSCs at a 1:4 dilution on cells reaching confluence and have demonstrated that the cells expand over at least 10 passages with similar kinetics of growth in all donors tested.”
Adult mesenchymal stem cells and cell-based tissue engineering (2002, background): “tissue [16], periosteum [17,18], synovial membrane [19], muscle [20], dermis [21], pericytes [22–24], blood [25], bone marrow [26], and most recently trabecular bone”
Exosomes derived from miR-140-5p-overexpressing human synovial mesenchymal stem cells enhance cartilage tissue regeneration and prevent osteoarthritis of the knee in a rat model (2017, background): “Synovial mesenchymal stem cells (SMSCs) were first isolated from the synovial membrane surrounding joints in 2001[15].”
Mesenchymal Stem Cell Secretome: Toward Cell-Free Therapeutic Strategies in Regenerative Medicine (2017, background): “They have also been isolated from other adult tissues such as adipose tissue [10], skin [11], lung [12], synovial membrane [13], dental pulp [14], nasal olfactory mucosa [15], breast milk [16], scalp tissue [17], muscle [18], periosteum [19], corneal limbus [20], peripheral blood [21], endometrial a”
Mesenchymal stem cells reside in virtually all post-natal organs and tissues (2006, background): “Mesenchymal stem cells have been conventionally isolated from bone marrow (Pittenger et al., 1999; Kopen et al., 1999) and, more recently, from some other tissues (Zuk et al., 2001; De Bari et al., 2001; Seo et al., 2004; Sabatini et al., 2005).”
Mesenchymal stromal cells: new directions. (2012, background): “…(Erices et al., 2000), umbilical cord perivascular cells (Sarugaser et al., 2005), umbilical cord Wharton’s jelly (Wang et al., 2004), dental pulp (Gronthos et al., 2000), amniotic fluid (Nadri and Soleimani, 2007), synovial membrane (De Bari et al., 2001), and breast milk (Patki et al., 2010).”
Stem cell-based tissue engineering with silk biomaterials. (2006, background): “One such example is MSCs, which can be isolated from a wide variety of tissues including bone marrow [116,117], periosteum [118,119], synovium [120], muscle [121–123], adipose tissue [124], lung [125–127], bone [128], deciduous teeth [129], dermis [130], and articular cartilage [131].”
Multipotent mesenchymal stromal cells obtained from diverse human tissues share functional properties and gene-expression profile with CD146+ perivascular cells and fibroblasts. (2008, background): “Multipotent mesenchymal stromal cells (MSC) are obtained from a variety of adult and fetal tissues, including cord blood [4], dental pulp [5], adipose tissue [6], skin [7], muscle [8], liver [9], synovium [10], pancreas [11], lung [12], amniotic fluid [13], placenta [14], peripheral blood [15], and ”
Mesenchymal stem cells: clinical applications and biological characterization. (2004, background): “For instance, De Bari et al. (2001) showed that MSCs isolated from the synovium as an adherent cell population were capable of differentiation into chondrocytes, osteocytes and adipocytes.”