Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering

By Jin Zhang, Yuliang Ma, S. Taylor, Roger Tsien. Proceedings of the National Academy of Sciences of the United States of America, 2001

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Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosis. (2010, background): “Curves were aligned on NEBD and the onset of anaphase as in (A). energy transfer (FRET) sensor for protein kinase A (PKA) (Zhang et al., 2001), where the donor and acceptor fluorescent proteins are linked by a phosphorylation site specific for the kinase and a phosphobinding domain (see Figure S1A a”
Development of an optimized backbone of FRET biosensors for kinases and GTPases (2011, methodology): “The prototype biosensor is based on the structure of a PKA activity sensor, AKAR3 (Allen and Zhang, 2006), which contained a consensus peptide of PKA phosphorylation (Zhang et al., 2001) and the phosphate binding domain of FHA1 (Figure 2A).”
Fluorescent proteins and their applications in imaging living cells and tissues. (2010, background): “The universality and relative simplicity of such a design have led to the development of numerous FRETbased sensors of this type, including GES for monitoring changes in the concentration of Ca(2) (130, 153, 262, 263, 291, 292, 295, 304, 307, 327, 434), cyclic nucleotides (174, 317, 318, 375, 445, 5”
Dissecting tumour pathophysiology using intravital microscopy (2002, background): “that are involved in tumour progression, and to assess their involvement in different signalling pathway”