Whole-brain functional imaging at cellular resolution using light-sheet microscopy

By Misha B. Ahrens, M. Orger, D. Robson, Jennifer M. Li, Philipp J. Keller. Nature Methods, 2013

1,305 citations recorded by the source corpus.

Subfields: Biophysics, Cognitive Neuroscience

View Paper: https://www.semanticscholar.org/paper/05c6b97f4b889b372f59eaa69a76ccc552cd8eec

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Citations in the corpus

From the neuron doctrine to neural networks (2015, methodology): “From the initial development of organic calcium 152 or voltage 153,154 indicators to the more recent genetically encoded indicators 155–158 , it has become possible to measure the activity of many — or, in some cases, most 42,159 — neurons in a neural circuit.”
Simultaneous Denoising, Deconvolution, and Demixing of Calcium Imaging Data (2016, positive): “Recent technical advances have enabled complete imaging of small cortical volumes (e.g., Cotton et al., 2013), and whole-brain imaging of small animals (Ahrens et al., 2013; Prevedel et al., 2014) at reasonable imaging rates.”
Exponential Family Embeddings (2016, methodology): “Consider the (calcium) expression of a large population of zebraﬁsh neurons (Ahrens et al., 2013).”
Fully Integrated Silicon Probes for High-Density Recording of Neural Activity (2017, positive): “We further sought to develop probes with (3) low noise, (4) resistance to movement artefacts or other interference, and (5) efficient data transmission by integrating low noise analogue amplifiers, multiplexers and digitizers into the probe base.”
Sensitive red protein calcium indicators for imaging neural activity (2016, background): “, 2013b) to populations of thousands of neurons (Ahrens et al., 2013; Peron et al., 2015).”
Modular Brain Networks (2015, background): “For example, clusters of highly correlated brain regions can be derived from optical recordings of neural activity (Ahrens et al. 2013) or from spike time series on multielectrode arrays (Shimono & Beggs 2015).”
Simultaneous multi-plane imaging of neural circuits (2016, positive): “Despite their high performance, wide-field imaging schemes such as light-sheet microscopy (Ahrens et al., 2013), light field microscopy (Prevedel et al., 2014), or swept confocally-aligned planar excitation (SCAPE) (Bouchard et al., 2015), suffer from light scattering in deep tissues and are thus…”
A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging (2016, methodology): “Spurred by the development of fast solid state cameras, wide-field methods such as wide-field microscopy (Ziv et al. 2013), light-field microscopy (Prevedel et al. 2014), and light-sheet microscopy (Holekamp et al. 2008, Ahrens et al. 2013) have been used to measure neural activity.”
Whole-brain activity mapping onto a zebrafish brain atlas (2015, background): “Spatial (rather than temporal) ICA was chosen for three reasons: (1) the relatively slow nature of the pERKactivity indicator, which will limit the temporal separation of signals; (2) the complex nature of the stimuli and experiences of the individual fish, which may not yield strictly temporally in”