Whole-brain activity mapping onto a zebrafish brain atlas
By Owen Randlett, C. Wee, Eva A Naumann, Onyeka Nnaemeka, David Schoppik, James E. Fitzgerald, R. Portugues, Alix M. B. Lacoste, Clemens Riegler, F. Engert and 1 more. Nature Methods, 2015
488 citations recorded by the source corpus.
In order to localize the neural circuits involved in generating behaviors, it is necessary to assign activity onto anatomical maps of the nervous system. Using brain registration across hundreds of larval zebrafish, we have built an expandable open-source atlas containing molecular labels and definitions of anatomical regions, the Z-Brain. Using this platform and immunohistochemical detection of phosphorylated extracellular signal–regulated kinase (ERK) as a readout of neural activity, we have developed a system to create and contextualize whole-brain maps of stimulus- and behavior-dependent neural activity. This mitogen-activated protein kinase (MAP)-mapping assay is technically simple, and data analysis is completely automated. Because MAP-mapping is performed on freely swimming fish, it is applicable to studies of nearly any stimulus or behavior. Here we demonstrate our high-throughput approach using pharmacological, visual and noxious stimuli, as well as hunting and feeding. The resultant maps outline hundreds of areas associated with behaviors.
Subfields: Cell Biology, Cellular and Molecular Neuroscience, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/9a0f1ea3daa0a471a45ef684763aceabda85a05d
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Citations in the corpus
- Mapping of brain activity by automated volume analysis of immediate early genes (2016, unknown): “In fact, whole brain activity mapping with IEGs without sectioning has been shown to be a powerful approach to investigate brain regions active in freely moving zebrafish larvae using image registration (Wee et al., 2015), but the extension of this approach to the much larger mammalian brain has not”
- TX-Ray: Quantifying and Explaining Model-Knowledge Transfer in (Un-)Supervised NLP (2019, methodology): “5We use the term ‘activation’ and its neuroscience analog ‘response’ synonymously, since both describe a neuron’s behavior under changing ‘stimuli’ and ‘environments’ [31] and since a like-minded methodology to TX-Ray has recently been used to analyze and quantify knowledge change during task learni”
- NeuroStorm: Accelerating Brain Science Discovery in the Cloud (2018, methodology): “Image Database For Researchers in the Cloud The BossDB platform was developed by the Johns Hopkins University Applied Physics Lab, and is used byNeuroData to store large image volumes.”