High-resolution genotyping by amplicon melting analysis using LCGreen.

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Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons. (2004, methodology): “In most cases, plots of fluorescence vs temperature were normalized as described previously (1, 7).”
Interspecific Hybridization between Eggplant and Wild Relatives from Different Genepools (2016, methodology): “Twelve SNP markers distributed through different linkage groups of the eggplant genetic map (Gramazio et al., 2014) were selected for genotyping the parentals and hybrids (Table 2) using the high-resolution melting technique (Wittwer et al., 2003).”
Molecular genetic tools for environmental monitoring of New Zealand's aquatic habitats, past, present and the future (2013, methodology): “…order to measure the amount of amplified product in real-time, QPCR uses either intercalating dyes such as SYBR Green (Becker et al. 1996), LCGreen (Wittwer et al. 2003), SYTO9 (Monis et al. 2005), EvaGreen (Wang et al. 2006) or highlyspecific probe-based detection systems, including TaqMan (Heid…”
Real-Time PCR (2005, methodology): “Several closed-tube systems have been developed that can be used in combination with melting analysis of PCR products to identify both heterozygous and homozygous sequence variants,([5]) and it has even been possible to develop triplex assays that use SYBR Green I and dissociation curves to identify”