A Nonviral Minicircle Vector for Deriving Human iPS Cells

By Fangjun Jia, Kitchener D. Wilson, Ning Sun, Deepak M. Gupta, Mei Huang, Zongjin Li, R. Robbins, M. Kay, M. Longaker, Joseph C. Wu. 2010

740 citations recorded by the source corpus.

Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.

Subfields: Molecular Biology

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Citations in the corpus

Reprogramming of mouse and human cells to pluripotency using mature microRNAs. (2011, background): “Most of the reprogramming methods reported so far involve introduction of genetic materials and thus run the risk that exogenous vector sequences can be integrated into the host genome (Okita et al., 2008; Kaji et al., 2009; Woltjen et al., 2009; Jia et al., 2010).”
Highly efficient reprogramming to pluripotency and directed differentiation of human cells using synthetic modified mRNA (2010, background): “Importantly, methods that rely on repeat administration of transient vectors, whether DNA or protein based, have so far shown very low iPSC derivation efficiencies (Jia et al., 2010; Kim et al., 2009; Okita et al., 2008; Stadtfeld et al., 2008; Yu et al., 2009; Zhou et al., 2009), presumably because”
Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors (2011, methodology): “Toward this end, several techniques have been used for obtaining integration and/or transgene-free iPSCs, including the use of plasmids (3, 4), the Cre/ loxP system (5, 6), adenoviruses (7), piggyBac (8, 9), minicircle vector (10), and proteins (11, 12).”
Senescence impairs successful reprogramming to pluripotent stem cells. (2009, methodology): “Human integration-free iPS cells were later generated by adenoviral infection (Zhou and Freed, 2009), transfection with self-replicating episomal vectors (Yu et al., 2009) or with polycistronic minicircle vectors (Jia et al., 2010).”
Induced pluripotency: history, mechanisms, and applications. (2010, background): “…into iPSCs with adenoviral vectors (Zhou and Freed 2009) and Sendai virus (Fusaki et al. 2009), as well as with polycistronic minicircle vectors (Jia et al. 2010) and self-replicating selectable episomes (Yu et al. 2009), albeit the latter system required the simultaneous overexpression of…”