HP1 Binds Specifically to Lys26-methylated Histone H1.4, whereas Simultaneous Ser27 Phosphorylation Blocks HP1 Binding*
By S. Daujat, U. Zeissler, T. Waldmann, N. Happel, Robert Schneider. 2005
247 citations recorded by the source corpus.
Histone lysine methylation can have positive or negative effects on transcription, depending on the precise methylation site. According to the “histone code” hypothesis these methylation marks can be read by proteins that bind them specifically and then regulate downstream events. Hetero-chromatin protein 1 (HP1), an essential component of heterochromatin, binds specifically to methylated Lys9 of histone H3 (K9/H3). The linker histone H1.4 is methylated on Lys26 (K26/H1.4), but the role of this methylation in downstream events remains unknown. Here we identify HP1 as a protein specifically recognizing and binding to methylated K26/H1.4. We demonstrate that the Chromo domain of HP1 is mediating this binding and that phosphorylation of Ser27 on H1.4 (S27/H1.4) prevents HP1 from binding. We suggest that methylation of K26/H1.4 could have a role in tethering HP1 to chromatin and that this could also explain how HP1 is targeted to those regions of chromatin where it does not colocalize with methylated K9/H3. Our results provide the first experimental evidence for a “phospho switch” model in which neighboring phosphorylation reverts the effect of histone lysine methylation.
Subfields: Molecular Biology
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In Molecular Biology
- K. Helin
- A. Bracken
- Diego Pasini
- Anna Portela
- K. Hansen
- Nikolaj Dietrich
- Manel Esteller
- Anke Sparmann
Citations in the corpus
- Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions. (2006, background): “2004), and since H1K26me3 can tether HP1 to chromatin (Daujat et al. 2005), this could add an additional layer of transcriptional repression on PcG target genes.”
- Epigenetic modifications and human disease (2010, background): “It does not form part of the nucleosome but binds to the linker DNA (that is, the DNA separating two histone complexes), sealing off the nucleosome at the location where DNA enters and leave”
- Polycomb silencers control cell fate, development and cancer (2006, background): “Methylated H1K26 can tether heterochromatin binding protein 1 (HP1) to chromati”