Detection of beta 2-adrenergic receptor dimerization in living cells using bioluminescence resonance energy transfer (BRET).

By S. Angers, A. Salahpour, E. Joly, S. Hilairet, D. Chelsky, M. Dennis, Michel Bouvier. Proceedings of the National Academy of Sciences of the United States of America, 2000

728 citations recorded by the source corpus.

Subfields: Cellular and Molecular Neuroscience, Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/01667973833cece62ca83a0f0d84975a4e6d8d6a

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cellular and Molecular Neuroscience

1. Arthur Christopoulos
2. Michel Bouvier
3. Bryan L. Roth
4. Brian K. Kobilka

In Molecular Biology

1. Arthur Christopoulos
2. Terry Kenakin
3. Bryan L. Roth
4. Michel Bouvier
5. S. Angers
6. Brian K. Kobilka

Citations in the corpus

• Dimerization: an emerging concept for G protein-coupled receptor ontogeny and function. (2002, background): “The first BRET study to document GPCR dimerization in living cells was carried out using fusion proteins that genetically link GFP and Renilla luciferase (Rluc) to the carboxyl terminus of the human β2-adrenergic receptor ( β2AR) (20).”
• PRESTO-TANGO: an open-source resource for interrogation of the druggable human GPCR-ome (2015, positive): “…described to quantify GPCR– β -arrestin interactions, including high-content screening (HCS) 17 , bioluminescence resonance energy transfer (BRET) 18 , enzyme complementation 19 and transcriptional activation following arrestin translocation (Tango) 20 , although none are routinely performed in…”
• G Protein-Coupled Receptor Allosterism and Complexing (2002, background): “Although originally identified in cellular membrane fragments, 2-adrenoceptor homodimerization has subsequently been demonstrated in vivo in intact cells (Angers et al., 2000, 2001).”
• Roles of G‐protein‐coupled receptor dimerization (2004, background): “Whereas several studies suggest that ligand binding can regulate the dimer by either promoting (Rodriguez-Frade et al, 1999; Angers et al, 2000; Rocheville et al, 2000a,b; Cornea et al, 2001; Horvat et al, 2001; Kroeger et al, 2001; Wurch et al, 2001; Patel et al, 2002; Zhu et al, 2002;…”