Detection of arbuscular mycorrhizal fungi (Glomales) in roots by nested PCR and SSCP (Single Stranded Conformation Polymorphism)

By R. Kjøller, S. Rosendahl. Plant and Soil, 2000

150 citations recorded by the source corpus.

Subfields: Cell Biology, Insect Science, Plant Science

View Paper: https://www.semanticscholar.org/paper/0096f783bd80c672a005bb87468af23f14ebb08e

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In Cell Biology

1. A. Roldán
2. M. Alguacil
3. J. Young
4. Jaikoo Lee
5. Sangsun Lee
6. I. Jakobsen
7. Z. Lozano
8. M. Campoy

In Insect Science

1. A. Roldán
2. M. Alguacil
3. J. Klironomos
4. J. Young
5. Jaikoo Lee
6. Sangsun Lee
7. I. Jakobsen
8. Z. Lozano

In Plant Science

1. M. Alguacil
2. A. Roldán
3. M. Hart
4. J. Klironomos
5. J. Trevors
6. J. Young
7. Jaikoo Lee
8. Sangsun Lee

Citations in the corpus

• Inoculation with arbuscular mycorrhizal fungi: the status quo in Japan and the future prospects (2002, background): “Single stranded conformation polymorphism (SSCP) of the large ribosomal subunit gene may be promising to discriminate isolates of several Glomus spp. (Kjøller and Rosendahl, 2000), while the sequence diversity in this region is very high (Clapp et al., 2001).”
• Phosphorus fertilisation management modifies the biodiversity of AM fungi in a tropical savanna forage system. (2010, methodology): “The single-stranded conformation polymorphism (SSCP) approach is a very sensitive and reproducible technique that has been applied successfully in studies in order to analyse the sequence diversity of AM fungi within roots (Kjøller and Rosendahl, 2000; Alguacil et al., 2009a,b).”
• A DGGE-cloning method to characterize arbuscular mycorrhizal community structure in soil (2008, background): “, 2001), singlestranded conformation polymorphism (SCCP) (Kjoller and Rosendahl, 2000), terminal RFLP (T-RFLP; Vandenkoornhuyse et al.”
• High functional diversity within species of arbuscular mycorrhizal fungi. (2004, methodology): “Recent development of molecular tools have confirmed the spore morphology-based taxonomy, but have also revealed intraspecific variations in, e.g. the ribosomal subunit sequences (e.g. Vandenkoornhuyse & Leyval, 1998; Kjøller & Rosendahl, 2000; Clapp et al ., 2001).”
• INTER‐ AND INTRASPECIFIC VARIATION OF THE PSEUDO‐NITZSCHIA DELICATISSIMA COMPLEX (BACILLARIOPHYCEAE) ILLUSTRATED BY RRNA PROBES, MORPHOLOGICAL DATA AND PHYLOGENETIC ANALYSES 1 (2006, methodology): “…were freeze dried, re-suspended in redistilled water and tested using UV-DAD HPLC at the University of Copenhagen (Lundholm et al. 2002a) and fluorescence-detection HPLC with precolumn FMOC derivatization at Kitasato University; detection limit was 0.3 ng/mL whole culture (Kotaki et al. 2000).”
• Improved PCR primers for the detection and identification of arbuscular mycorrhizal fungi. (2008, methodology): “PCR primers for subgroups or certain target taxa of AMF are relatively easy to design and have been used successfully in several studies (Sanders et al., 1995; Bago et al., 1998; Lanfranco et al., 1999; Kjøller & Rosendahl, 2000; Redecker, 2000; Gamper & Leuchtmann, 2007).”
• Bacterial endophytes and their interactions with hosts. (2006, background): “…author: Esperanza Martínez-Romero; E-mail: emartine@ccg.unam.mx molecular analysis based on the sequence of ribosomal genes (Chelius and Triplett 1999; Jacquot et al. 2000; Kjoller and Rosendahl 2000) and other genes, such as those for actin or elongation factors (Helgason et al. 2003).”
• Methods of studying soil microbial diversity. (2004, background): “, 2000) and AMF species in roots (Simon et al., 1993; Kjoller and Rosendahl, 2000).”