Improved PCR primers for the detection and identification of arbuscular mycorrhizal fungi.
By Jaikoo Lee, Sangsun Lee, J. Young. FEMS Microbiology Ecology, 2008
802 citations recorded by the source corpus.
Subfields: Cell Biology, Insect Science, Plant Science
View Paper: https://www.semanticscholar.org/paper/4173343272649c5d5c26f0a76d20eaa58ae75c06
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Citations in the corpus
- Nitrogen deposition and precipitation induced phylogenetic clustering of arbuscular mycorrhizal fungal communities (2017, methodology): “The primer pair AML1 and AML2 (Lee et al., 2008) were used in the first PCR to amplify the small submit ribosomal DNA (SSU rDNA) region.”
- The online database MaarjAM reveals global and ecosystemic distribution patterns in arbuscular mycorrhizal fungi (Glomeromycota). (2010, methodology): “These groupings correspond roughly to the species level (Lee et al., 2008) or slightly above (see the next paragraph).”
- Root-colonizing and soil-borne communities of arbuscular mycorrhizal fungi in a temperate forest understorey1 (2014, methodology): “Glomeromycota sequences were amplified from root DNA extracts using the small-subunit (SSU) rRNA gene primers NS31 and AML2 (Simon et al. 1992; Lee et al. 2008).”
- Phylogenetic structure of arbuscular mycorrhizal community shifts in response to increasing soil fertility (2015, methodology): “Briefly, partial 18S rRNA gene sequences of AM fungi were amplified from soil DNA via a nested PCR, using GeoA2-Geo11 (Schwarzott and Schüßler, 2001) and NS31-AML2 (Simon et al., 1992; Lee et al., 2008) as the first and second primer combinations, respectively.”
- Fungal community analysis by high-throughput sequencing of amplified markers – a user's guide (2013, methodology): “Alternatively, a variable region of the SSU is amplified with the primers NS31AM1, and the variable D2 region of the LSU is amplified with the primer FLR3 (aka glo454) in combination with either FLR4 or NDL22 (aka TW13) (van Tuinen et al., 1998; Gollotte et al., 2004; Lee et al., 2008; € Opik et al.”
- Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples (2018, methodology): “The universal eukaryote primer NS31 and the Glomeromycota specific primer AML2 (Lee et al. 2008) have been increasingly adopted for 454-pyrosequencing.”
- Plant type differently promote the arbuscular mycorrhizal fungi biodiversity in the rhizosphere after revegetation of a degraded, semiarid land (2010, methodology): “Two ml of several dilutions (1/10, 1/20, 1/50 and 1/100) from the first PCR were used as template DNA in a second PCR reaction performed using the specific primers AML1 and AML2 (Lee et al., 2008).”
- Six-year fertilization modifies the biodiversity of arbuscular mycorrhizal fungi in a temperate steppe in Inner Mongolia (2014, methodology): “Partial sequences of the SSU rRNA genes were amplified using a nested PCR with a first glomeromycotan-specific primer pair AML1 and AML2 (Lee et al., 2008), and a second primer combination of universal eukaryotic primer NS31 (Simon et al., 1992) as forward primer and the primer AM1 (Helgason et…”
- Changes in the composition and diversity of AMF communities mediated by management practices in a Mediterranean soil are related with increases in soil biological activity (2014, methodology): “Two ml of several dilutions (1/10, 1/20, 1/50 and 1/100) from the first PCR were used as template DNA in a second PCR reaction performed using the specific primers AML1 and AML2 (Lee et al., 2008).”
- Phylogenetic reference data for systematics and phylotaxonomy of arbuscular mycorrhizal fungi from phylum to species level. (2012, methodology): “Several SSU-targeting PCR primers (e.g. Simon et al., 1992; Helgason et al., 1998; Lee et al., 2008) that amplify fragments of c.”