Cells lying on a bed of microneedles: An approach to isolate mechanical force

By John L. Tan, J. Tien, Dana M. Pirone, D. S. Gray, Kiran Bhadriraju, Christopher S. Chen. Proceedings of the National Academy of Sciences of the United States of America, 2003

2,017 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/0054ef526db67ea4845178f8e7f5727faf568390

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Citations in the corpus

Mechanical control of stem cell differentiation (2008, background): “In parallel, the actin cytoskeleton contributes to the mechanical forces within the system due to the pulling action of myosin motors (principally non-muscle myosin II) on actin filaments, which result in the production of tensile forces against the extracellular matrix (Pelham and Wang, 1999; Balab”
The biomechanics toolbox: experimental approaches for living cells and biomolecules (2003, background): “It has been shown [4] that the traction forces increased as the FAC size increased, at least as this FAC size was quantified through fluorescence of a particular ECM protein.”
A model for the contractility of the cytoskeleton including the effects of stress-fibre formation and dissociation (2007, background): “Responses of a square cell on four supports Experiments to probe the forces generated by a cell on a bed of micro-needles (Tan et al. 2003) have motivated the two-dimensional plane stress problem illustrated in figure 13.”
Physical traits of cancer (2020, methodology): “P. P. Provenzano, D. R. Inman, K. W. Eliceiri, S. M. Trier, P. J. Keely, Contact guidance mediated three-dimensional cell migration is regulated by Rho/ROCK-dependent matrix reorganization.”
High resolution traction force microscopy based on experimental and computational advances. (2008, unknown): “0 Gijðx;Px91 ;Px92ÞPx9i dPx91 dPx92 : (19)”
Fibroblast adaptation and stiffness matching to soft elastic substrates. (2007, result): “The response of the fibroblast to increased substrate stiffness in the range from 500 Pa to 10 kPa involves a reorganization of the cytoskeleton to produce a more organized system of filament bundles and increasing amounts of cross-linked actin filaments as seen in Fig. 5 and in previous reports (5,”
Adhesion-dependent cell mechanosensitivity. (2003, background): “…al. 2001 2–10 CAR (fibroblasts) Polyacrylamide gel with embedded beads Munevar et al. 2001 2–10 3T3 (fibroblasts) Polyacrylamide gel with embedded beads Tan et al. 2003 4.8 BPASMC (smooth elastomeric (PDMS) posts muscle) Burton et al. 1999 2–15 Fish Keratocytes Wrinkling silicone rubber…”
Mechanical Integration of Actin and Adhesion Dynamics in Cell Migration (2010, background): “By contrast, in quiescent fibroblasts, traction force is proportional to the FA area (Balaban et al. 2001, Goffin et al. 2006, Tan et al. 2003).”
A Review of Cell Adhesion Studies for Biomedical and Biological Applications (2015, background): “Upon binding, integrins cluster into FA complexes that transmit adhesive and traction forces [25,26].”