Three-Dimensional Super-resolution Imaging of Thick Biological Samples

By Alipasha Vaziri, Jianyong Tang, Hari Shroff, C. Shank. Microscopy and Microanalysis, 2009

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Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function (2009, methodology): “Importantly, several of these techniques have recently been pushed to 3 dimensions by using astigmatism (19), multiplane methods (20), and 2-photon activation by temporal focusing (21) to quantify the z position of the emitters.”