M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods.
By I. Boutin-Ganache, M. Raposo, M. Raymond, C. Deschepper. BioTechniques, 2001
681 citations recorded by the source corpus.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/ff9ed33f8a7d8e5e0963e33699a50d5865710503
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In Molecular Biology
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Citations in the corpus
- Genetic linkage maps of Populus nigra L. including AFLPs, SSRs, SNPs, and sex trait (2007, methodology): “The PCR reactions were carried out using the M13-tailed method (Oetting et al. 1995; Boutin-Ganache et al. 2001).”
- Isolating microsatellite DNA loci. (2005, methodology): “After editing, primers for PCR are designed from the sequences flanking DNA using standard methodology or a three-primer system (Boutin-Ganache et al., 2001); see 50PrimerTags3.”
- Microsatellite DNA loci from the Diamondback terrapin (Malaclemys terrapin) (2003, methodology): “Inclusion of the 5′-tag allows use of a third primer in the PCR (M13R or CAG) that is fluorescently labelled for detection on the ABI 377 (cf. Boutin-Ganache et al. 2001).”