Isolating microsatellite DNA loci.

By Travis C. Glenn, Nancy A. Schable. Methods in Enzymology, 2005

902 citations recorded by the source corpus.

Subfields: Ecology, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/a125ca2e8595108356f687cfd4933f0a9e5aad01

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Citations in the corpus

Methods for Development of Microsatellite Markers: An Overview (2014, positive): “, 2010) or restriction enzymes (Brown et al., 1995; Chen et al., 1995; Edwards et al., 1996; Prochazka., 1996; Refseth et al., 1997; Fischer and Bachmann, 1998; Hamilton et al., 1999; Glenn and Schable, 2005; Nunome et al., 2006) or nebulisation (Kumpatla et al.”
msatcommander: detection of microsatellite repeat arrays and automated, locus‐specific primer design (2008, background): “2001; Glenn & Schable 2005) has helped greatly reduce costs associated with this process, the careful addition of 5 ′ -tails before polymorphism testing introduces additional time demands.”
Novel SSR Markers for Polymorphism Detection in Pigeonpea (Cajanus spp.) (2010, methodology): “Construction of SSR enriched library: To isolate new SSR loci, a SSR enriched library was constructed from Asha genotype (ICPL 87119) using a bead capture enrichment protocol (Glenn and Schable 2005).”
Using nuclear gene data for plant phylogenetics: Progress and prospects II. Next‐gen approaches (2015, background): “Initially, their development required a significant investment of time and resources (including DNA enrichment, cloning and Sanger sequencing steps) just for production of one to two dozen primer pairs that generated polymorphic fragment profiles (Glenn & Schable, 2005).”
Microsatellites for ecologists: a practical guide to using and evaluating microsatellite markers. (2006, background): “However, the process of isolating new microsatellite markers has become faster and less expensive, which substantially reduces the failure rate and/or cost of new marker isolation in many cases (Glenn & Schable 2005).”
Plant Parentage, Pollination, and Dispersal: How DNA Microsatellites Have Altered the Landscape (2010, background): “As improved protocols emerged, notably the use of enriched libraries where microsatellite-containing DNA fragments are selectively cloned (Glenn and Schable, 2005; Zane et al., 2002), the development step has been reduced from months to weeks.”
Non-coding nuclear DNA markers in phylogenetic reconstruction (2009, methodology): “This technique, which was subsequently modified by Glenn and Schable (2005), is the most widely used method in microsatellite isolation.”