Effects of manipulation of pyruvate decarboxylase and alcohol dehydrogenase levels on the submergence tolerance of rice
By Musrur Rahman, A. Grover, W. Peacock, E. Dennis, M. Ellis. 2001
101 citations recorded by the source corpus.
A transgenic approach was taken to manipulate the levels of pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) in rice, in order to investigate whether alteration of ethanol fermentation can affect anaerobic tolerance. A line transformed with an antisense Adh1 construct had only 4–8% of the ADH activity of untransformed plants. This line showed reduced ethanol production and coleoptile growth under anoxia. Mature plants had reduced survival when submerged in water and exposed to anoxia, suggesting that ADH plays an essential role in seed germination and plant survival in the absence of O2. A transgenic line transformed with a cotton Adh2 cDNA in the sense orientation relative to a constitutive promoter, showed 3–4-fold more ADH activity than either untransformed controls, or a flooding-tolerant rice variety (FR13A), both in air and under hypoxia. However, ethanol production by this line was only slightly higher than that of untransformed controls, and there was no increase in survival following anoxia treatments. Three independent transgenic lines containing the ricePdc1 cDNA driven by an anaerobically-inducible promoter (6XARE) showed an increase in PDC1 polypeptide in shoots, but not in roots or endosperm. A moderate increase in PDC activity and ethanol production was observed in shoots of these lines under anaerobic conditions, as well as decreased survival of shoots when submerged and exposed to anoxia. F1 plants containing both the PDC and ADH constructs showed levels of anoxia-tolerance similar to those of untransformed plants. These results suggest that over-production of PDC may be toxic to rice plants because of increased acetaldehyde. Consistent with this view, acetaldehyde levels were appreciably higher in plants over-producing PDC, compared with untransformed plants, or hybrid lines containing both the PDC and ADH constructs.
Subfields: Ecology, Plant Science
View Paper: https://www.semanticscholar.org/paper/ff55bd83e74abcfcd2985b3df20090671469a64b
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In Ecology
In Plant Science
- J. Bailey-Serres
- T. Fukao
- S. Agarwal
- A. Grover
- T. Setter
- Pamela C. Ronald
- I. Waters
- Laurentius A. C. J. Voesenek
Citations in the corpus
- A Variable Cluster of Ethylene Response Factor–Like Genes Regulates Metabolic and Developmental Acclimation Responses to Submergence in Rice[W] (2006, background): “In fact, adh and pdc loss-of-function mutants inmaize, rice, andArabidopsis thaliana succumb rapidly to low oxygen and submergence stress, confirming that ethanolic fermentation is necessary for acclimation to the transient stress (Schwartz, 1969; Rahman et al., 2001; Baxter-Burrell et al., 2003; Is”
- Molecular Biology, Biotechnology and Genomics of Flooding-Associated Low O2 Stress Response in Plants (2006, methodology): “In contrast to this study, Rahman et al. (2001) showed that rice transgenics over-expressing rice pdc1 exhibit lower survival after submergence in anoxic water than that of untransformed plants and submergence intolerant cultivar IR42.”
- Review of prospects for germplasm improvement for waterlogging tolerance in wheat, barley and oats (2003, background): “…(1) differential expression of PDC in shoot and root tissue, (2) the use of different constitutive (35S; Quimio et al., 2000) and inducible (6XARE; Rahman et al., 2001) promoters; or (3) less severe but longer term treatment conditions used by Quimio et al. (2000) (14 d submergence under hypoxic…”