Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR.

By S. Henry, Ezékiel Baudoin, Juan C. López-Gutiérrez, F. Martin-Laurent, A. Brauman, L. Philippot. Journal of Microbiological Methods, 2004

678 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology, Pollution

View Paper: https://www.semanticscholar.org/paper/fceb4fe6bd37ac18a3b2df807cba6feaf4243292

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

1. L. Philippot
2. D. Bru
3. Christopher M. Jones
4. S. Hallin
5. K. Deiglmayr
6. E. Kandeler
7. D. Tscherko
8. Janet Andert

In Molecular Biology

1. S. Hallin
2. D. Bru
3. K. Butterbach‐Bahl
4. Christopher M. Jones
5. Peter M. Groffman
6. T. Kana
7. M. Firestone
8. L. Philippot

In Pollution

1. L. Philippot
2. Christopher M. Jones
3. D. Bru
4. S. Hallin
5. K. Butterbach‐Bahl
6. Peter M. Groffman

Citations in the corpus

• Abundance of narG, nirS, nirK, and nosZ Genes of Denitrifying Bacteria during Primary Successions of a Glacier Foreland (2006, background): “rRNA or the narG, nirK, nirS, or nosZ gene (6, 7, 12).”
• Quantitative Detection of the nosZ Gene, Encoding Nitrous Oxide Reductase, and Comparison of the Abundances of 16S rRNA, narG, nirK, and nosZ Genes in Soils (2006, methodology): “(8) but 1 to 2 orders of magnitude lower than those observed by Qiu et al.”
• Methods for measuring denitrification: diverse approaches to a difficult problem. (2006, methodology): “…are used to assess abundance such as direct DNA probing (Linne von Berg and Bothe 1992), quantitative PCR or real-time PCR (Gruntzig et al. 2001, Henry et al. 2004), which measure gene copies and relates that back to cell numbers, or T-RFLP (terminally labeled restriction-length fragment…”
• Importance of denitrifiers lacking the genes encoding the nitrous oxide reductase for N2O emissions from soil (2011, methodology): “Fragments of the nirK, nirS and nosZ genes encoding the copper and cd1 nitrite reductases and the nitrous oxide reductases, respectively, were amplified using primers and thermal cycling conditions described previously (Henry et al., 2004, 2006).”
• Niche construction by the invasive Asian knotweeds (species complex Fallopia): impact on activity, abundance and community structure of denitrifiers and nitrifiers (2011, positive): “Quantification of denitrifiers The determination of denitrifiers abundance was done by quantitative PCR targeting the nirK gene function (Henry et al. 2004) (encoding for Cu-nitrite reductase).”