Quantitative Detection of the nosZ Gene, Encoding Nitrous Oxide Reductase, and Comparison of the Abundances of 16S rRNA, narG, nirK, and nosZ Genes in Soils

By S. Henry, D. Bru, B. Stres, S. Hallet, L. Philippot. Applied and Environmental Microbiology, 2006

976 citations recorded by the source corpus.

Subfields: Ecology, Environmental Engineering, Pollution

View Paper: https://www.semanticscholar.org/paper/f93d4726f134b0b2d877b476b4cc51d29f43ac62

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Citations in the corpus

Plant litter diversity increases microbial abundance, fungal diversity, and carbon and nitrogen cycling in a Mediterranean shrubland (2017, methodology): “Standard solution of bacterial 16S rDNA 219 gene was generated from a pGEM-T cloned 16S rDNA gene of a soil representative strain 220 (Henry et al., 2006).”
Unexpected nondenitrifier nitrous oxide reductase gene diversity and abundance in soils (2012, background): “PCR assays confirmed that commonly used nosZ-targeted primer sets including Nos661F/Nos1773R (52), NosZ-F/nosZ1622R (51, 53), nosZ1F/nosZ1R, and nosZ2F/nosZ2R (49) are not comprehensive and fail to amplify the atypical nosZ genes.”
FunGene: the functional gene pipeline and repository (2013, methodology): “…database (Spain et al., 2007; Wertz et al., 2009; Bull et al., 2012; Lin et al., 2012; Fang et al., 2013), a primer designing/testing tool (Henry et al., 2006; Philippot and Hallin, 2006; Bru et al., 2007; Leigh et al., 2007; Stedtfeld et al., 2007; Jones et al., 2008, 2011, 2012; Stres…”
Loss in microbial diversity affects nitrogen cycling in soil (2013, methodology): “Briefly, 20 cycles of amplification were performed with the nosZ1 (50-WCSYTGT TCMTCGACAGCCAG-30) and nosZ2 (50-ACSSKSA CCTTSTTGCCGTYGC-30) primers previously described (Henry et al., 2006).”
Metagenomic reconstruction of nitrogen cycling pathways in a CO2-enriched grassland ecosystem (2017, methodology): “, 2006), and nosZ (nitrous oxide reductase) (Bru et al., 2011; Henry et al., 2006; Kandeler et al., 2006), and were carried out by amplification-based approaches.”
Importance of denitrifiers lacking the genes encoding the nitrous oxide reductase for N2O emissions from soil (2011, methodology): “Furthermore, quantification of denitrification genes in soils commonly show a lower abundance of bacteria having the nosZ gene compared with those having the other genes encoding the different denitrifying reductases (Henry et al., 2006; Babic et al., 2008; Hallin et al., 2009; Bru et al., 2010).”
The unaccounted yet abundant nitrous oxide-reducing microbial community: a potential nitrous oxide sink (2012, methodology): “Environmental studies have commonly shown a 2–10 times lower abundance of denitrifiers harboring the nosZ gene compared with those having the genes encoding the preceding denitrifying reductases in the pathway (Henry et al., 2006; Hallin et al., 2009; Bru et al., 2010).”
Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology. (2009, methodology): “Used to quantify genes from a range of soil types Henry et al. (2006) Nitric oxide reduction norB Two SYBR green primer sets targeting the cytochrome c electron donor (cNOR) form of the enzyme; designed from cultured soil isolates.”