Real-time PCR quantification of precursor and mature microRNA.

By Thomas D. Schmittgen, E. J. Lee, Jinmai Jiang, A. Sarkar, Liuqing Yang, T. Elton, Caifu Chen. Methods, 2008

619 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/fbde1b3ce6f3d07f2e6d293671be02c50757f3aa

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In Cancer Research

In Molecular Biology

Citations in the corpus

Microvesicles released from human renal cancer stem cells stimulate angiogenesis and formation of lung premetastatic niche. (2011, background): “miRNAs with raw Ct values greater than 35 in both preparations were not included in the analysis, as they were considered nonspecific (18, 19).”
Detection of microRNA Expression in Human Peripheral Blood Microvesicles (2008, methodology): “At the time of the study, commercially available primers were available for 420 mature human miRNAs. We used these looped primers to profile 420 mature miRNAs by real-time PCR using an Applied Biosystems 7900HT real-time PCR instrument equipped with a 384 well reaction plate. RNA (500 ng) was conver”
Microvesicles Derived from Adult Human Bone Marrow and Tissue Specific Mesenchymal Stem Cells Shuttle Selected Pattern of miRNAs (2010, background): “Statistical analysis Since in qRT-PCR, Ct scores greater than 35 are considered non-specific [67], miRNAs that had a raw Ct value greater than 35, conserved in both preparations of MSCs, HLSCs or their corresponding MVs, were not included in the final data analysis.”
Plant microRNAs: new players in functional genomics (2013, positive): “…TaqMan probes with mature miRNA-specific stem-loop RT primer increases the sensitivity and specificity of the methods; therefore, stem-loop RT based TaqMan is now considered the gold standard among the miRNA detection methods (Mestdagh et al. 2008; Schmittgen et al. 2008; Becker et al. 2010).”
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper (2017, methodology): “accumulate for hours/days.[212,213] Therefore, detecting changes in precursor miRNAs in destination cells may not be a reliable method to control for spurious”
A novel class of small RNAs: tRNA-derived RNA fragments (tRFs). (2009, methodology): “To circumvent this problem, we used quantitative RT–PCR (qRT–PCR) detection in a method similar to the detection of miRNAs (for review, see Schmittgen et al. 2008).”
Circulating miRNAs are correlated with tumor progression in prostate cancer (2011, methodology): “After sample outlier removal, a filtering step was applied, wherein Ct values 35 were considered as nonspecific.(17) miRNAs, for which >70% of all values were nonspecific, were excluded from the analysis.”