Sequence-specific detection of individual DNA strands using engineered nanopores

By S. Howorka, S. Cheley, H. Bayley. Nature Biotechnology, 2001

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Continuous base identification for single-molecule nanopore DNA sequencing. (2009, methodology): “Cysteine mutants of α -haemolysin The mutant α HL pores used in this study were expressed, assembled and purified as described previousl”
Nanopore-based Fourth-generation DNA Sequencing Technology (2015, methodology): “The engineered transmembrane protein pores of a-HL have been used as stochastic sensing elements for the identification and quantification of a wide variety of substances, including cations [66], anions [11], organic molecules [67], enantiomers [68], chemical and biological terrorism agents [69–72],”
Solid-state nanopores. (2007, background): “Similarly, the addition of a nucleotide oligomer makes it possible to probe the hybridization of complementary DNA: if the sequences match, duplex binding will occur and the DNA translocation will be halted until, after a while, the DNA is unzipped and translocates through the por”
Stochastic sensors inspired by biology (2001, background): “When single-stranded oligonucleotides are covalently attached within the large cavity of the pore, sequence-specific duplex formation can be detecte”
Fabrication of solid-state nanopores with single-nanometre precision (2003, background): “Notable examples include α-hemolysin protein nanopores in lipid membrane”
Transport phenomena in nanofluidics (2008, background): “Howorka et al., 2001; Wang and Branton, 2001; Meller, 2003; Ashkenasy et al., 2005; Chan, 2005".”