A de novo designed protein–protein interface

By Po-Ssu Huang, John James Love, Stephen L. Mayo. 2007

102 citations recorded by the source corpus.

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In Molecular Biology

Citations in the corpus

A de novo protein binding pair by computational design and directed evolution (2011, methodology): “Computational Approach Previous approaches to de novo protein interface design by us and others (Huang et al., 2007), involved first positioning the two component proteins near one another in space via computational docking.”
Emerging themes in the computational design of novel enzymes and protein–protein interfaces (2013, background): “Initial successes were demonstrated in designing low-afﬁnity binding pairs through computational docking followed by design to generate a heterodimer comprising two variants of a single nat-ural protein [43] and a designed protein that bound a natural target protein [44].”
Protein Engineering by Combined Computational and In Vitro Evolution Approaches. (2016, background): “For this reason, the initial computationally designed NBDs suffered from low specificity and low affinity [41].”
RosettaRemodel: A Generalized Framework for Flexible Backbone Protein Design (2011, background): “Computational protein design tools to date have been useful for engineering proteins with a wide range of functions, including DNA binding [1,2,3,4], co-factor binding [5], catalysis [6,7,8,9], fluorescence spectral change [10], peptide-protein specificity [11,12], and protein-protein interaction [1”
Computational design of a protein-based enzyme inhibitor. (2013, unknown): “…have generated protein pairs that have been shown to interact experimentally, though the structures of the complexes either have not been solved [4] or have been found to be different from the original design conception [5], highlighting the challenges to designing specific and accurate…”
AbDesign: an algorithm for combinatorial backbone design guided by natural conformations and sequences (2015, unknown): “1,2,6,7,56”
Achievements and Challenges in Computational Protein Design. (2017, methodology): “Notably, in 2007 the Mayo lab used ORBIT [18] to design 13 and 11 residues on two monomer variants of streptococcal protein G—β1 domain (Gβ1) that were designed to heterodimerize [60].”
Practically Useful: What the Rosetta Protein Modeling Suite Can Do for You (2010, background): “So far, the most successful attempts at de novo interface design have been relatively modest, focusing on small proteins and yielding micromolar affinity ( 20 , 81 ).”