Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi.

By I. Maeda, Yuji Kohara, Masayuki Yamamoto, A. Sugimoto. Current Biology, 2001

721 citations recorded by the source corpus.

Subfields: Aging, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/f9328f5540096d433f041b35262561907ff7d984

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Aging

1. James P. McCarter
2. Ronald H. A. Plasterk
3. J. Ahringer
4. F. Simmer
5. R. Kamath
6. Andrew G. Fraser
7. Sam Griffiths-Jones
8. Matthew W. Hahn

In Genetics

1. James P. McCarter
2. Elaine R. Mardis
3. Richard K. Wilson
4. Richard Durbin
5. Marco A. Marra
6. Jason E. Stajich
7. Matthew W. Hahn
8. Andrew D. Kern

In Molecular Biology

1. James P. McCarter
2. Elaine R. Mardis
3. Richard Durbin
4. J. Ahringer
5. R. Kamath
6. Andrew G. Fraser
7. David E. Hill
8. Nansheng Chen

Citations in the corpus

• Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library. (2004, background): “Most of the previous RNAi studies were done by initiating RNAi on young adults (Fraser et al. 2000; Gonczy et al. 2000; Piano et al. 2000, 2002; Maeda et al. 2001; Zipperlen et al. 2001; Kamath et al. 2003; Simmer et al. 2003).”
• Genome-Wide RNAi of C. elegans Using the Hypersensitive rrf-3 Strain Reveals Novel Gene Functions (2003, methodology): “elegans using this RNAi library or other techniques (Gönczy et al. 2000; Maeda et al. 2001; Dillin et al. 2002; Piano et al. 2002; Ashrafi et al. 2003; Lee et al. 2003; Pothof et al. 2003).”
• Comparative genomics of centrality and essentiality in three eukaryotic protein-interaction networks. (2005, methodology): “…the knock-out data from Giaever et al. (2002) to ask whether a gene was essential; for worm we used the RNAi knock-down data from multiple studies (Maeda et al. 2001; Kamath et al. 2003) to assess embryonic lethality; and for fly we found all the genes known to have lethal mutants that were…”
• Dangerous liaisons: The symbiosis of entomopathogenic nematodes and bacteria (2006, background): “Soaking is feasible for large-scale screens (Maeda et al., 2001), although it is less eYcient than feeding because RNA must be prepared from each construct.”
• Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans (2005, background): “Cortical dynamics Little/no cortical ruffling or pseudocleavage furrow, or excessive cortical activity at two-cell stage 19 (6) Rho, transcription Cortical structure Y51H4A.”
• Molecular Approaches Toward Resistance to Plant-Parasitic Nematodes (2008, methodology): “…in C. elegans (Fire et al. 1998) . dsRNA microinjection, soaking, and feeding protocols have been used to knock down expression of all C. elegans genes and phenotypic effects have been observed for several thousand (Fraser et al. 2000 ; Gonczy et al. 2000 ; Kamath et al. 2003 ; Maeda et al. 2001) .”