A concise guide to cDNA microarray analysis.

By P. Hegde, R. Qi, K. Abernathy, C. Gay, S. Dharap, R. Gaspard, J. E. Hughes, E. Snesrud, N. Lee, John Quackenbush. BioTechniques, 2000

1,014 citations recorded by the source corpus.

Microarray expression analysis has become one of the most widely used functional genomics tools. Efficient application of this technique requires the development of robust and reproducible protocols. We have optimized all aspects of the process, including PCR amplification of target cDNA clones, microarray printing, probe labeling and hybridization, and have developed strategies for data normalization and analysis.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/f8c03736ca25faab8f36a9f00550c326da142d90

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In Molecular Biology

1. Shrikant M. Mane
2. S. Long
3. D. Ort
4. Carl Bernacchi
5. T. Sharkey

Citations in the corpus

• Microarray results: how accurate are they? (2002, methodology): “cDNA microarrays are created by spotting amplified cDNA fragments in a high density pattern onto a solid substrate such as a glass slide [1, 2 ].”
• Transcript expression profiles of Arabidopsis thaliana grown under controlled conditions and open-ai (2004, methodology): “Total RNA, 200 mg per sample, was labeled using the indirect labeling procedure as described by Hedge et al. (2000) with some modifications.”