A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila

By X. Morin, R. Daneman, M. Zavortink, W. Chia. Proceedings of the National Academy of Sciences of the United States of America, 2001

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The Carnegie Protein Trap Library: A Versatile Tool for Drosophila Developmental Studies (2007, positive): “…genome annotation is highly supported by experimental evidence (Misra et al. 2002); hence the frequency of these discrepancies was surprising, but a similar outcome has been reported in previous protein trap screens using both yeast (RossMacdonald et al. 1999) and Drosophila (Morin et al. 2001).”
Genetic manipulation of genes and cells in the nervous system of the fruit fly (2011, methodology): “Protein trapping was pioneered with the green fluorescent protein that was incorporated in a P element screen, resulting in some protein trap insertions (Morin et al., 2001;Clyne et al., 2003;Rolls et al., 2007).”
C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression (2003, background): “melanogaster supported the notion that 44% of its genes have not yet been predicte”
Polarity and intracellular compartmentalization of Drosophila neurons (2007, background): “The bel, Pdi, and Map205 genes all contain Wee-P [22] insertions, and the Jupiter line was previously described [23].”
MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes (2011, background): “When the protein trap is inserted in a coding intron in the appropriate orientation and reading frame, it reveals the protein's expression patter”