MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes
By K. Venken, Karen L. Schulze, Nele A. Haelterman, Hongling Pan, Yuchun He, M. Evans-Holm, Joseph W. Carlson, R. Levis, A. Spradling, R. Hoskins and 1 more. Nature Methods, 2011
666 citations recorded by the source corpus.
We demonstrate the versatility of a collection of insertions of the transposon Minos-mediated integration cassette (MiMIC), in Drosophila melanogaster. MiMIC contains a gene-trap cassette and the yellow+ marker flanked by two inverted bacteriophage ΦC31 integrase attP sites. MiMIC integrates almost at random in the genome to create sites for DNA manipulation. The attP sites allow the replacement of the intervening sequence of the transposon with any other sequence through recombinase-mediated cassette exchange (RMCE). We can revert insertions that function as gene traps and cause mutant phenotypes to revert to wild type by RMCE and modify insertions to control GAL4 or QF overexpression systems or perform lineage analysis using the Flp recombinase system. Insertions in coding introns can be exchanged with protein-tag cassettes to create fusion proteins to follow protein expression and perform biochemical experiments. The applications of MiMIC vastly extend the D. melanogaster toolkit.
Subfields: Molecular Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/4f819d6648eee61e1f696e8031d5424e691819de
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
- Julie H. Simpson
- Hugo J. Bellen
- K. Venken
- S. Zipursky
- C. Tsui
- Yi Chen
- Aljoscha Nern
- Matthew Y. Pecot
In Plant Science
Citations in the corpus
- Genetic manipulation of genes and cells in the nervous system of the fruit fly (2011, background): “Another system is known asMiMIC (minos-mediated integration cassette) (Venken et al., 2011) (Figure 2C).”
- Cell-type Specific Labeling of Synapses in vivo through Synaptic Tagging with Recombination (STaR) (2014, methodology): “, 2006), a variety of additional methods are available to generate STaR-based markers, such as Minos-mediated integration cassette (MiMIC) insertions (Venken et al., 2011) and site-specific recombination through cassette exchange (Pecot et al.”