Effects of sample processing on the detection rate of environmental DNA from the Common Carp (Cyprinus carpio)

By T. Takahara, Toshifumi Minamoto, Hideyuki Doi. 2015

111 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology, Nature and Landscape Conservation

View Paper: https://www.semanticscholar.org/paper/f600c59c1c32df87b5ae72e3f4b14555fbcaf3db

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In Ecology

In Molecular Biology

In Nature and Landscape Conservation

Citations in the corpus

Environmental DNA - An emerging tool in conservation for monitoring past and present biodiversity (2015, background): “River Crustacean, mussel Switzerland Present Pilliod et al. (2014) Factors influencing detection of eDNA from a streamdwelling amphibian Streams Amphibian (idaho giant salamander) Idaho, USA Present Tréguier et al. (2014) Environmental DNA surveillance for invertebrate species: advantages and techni”
Monitoring the near-extinct European weather loach in Denmark based on environmental DNA from water samples (2015, methodology): “Takahara et al. (2015) found that using smaller volumes of template (2 l L instead of 5 l L), led to higher qPCR detection probabilities for the common carp ( Cyprinius carpo ), likely due to inhibition when using large volumes.”
Efficacy of Environmental DNA to Detect and Quantify Brook Trout Populations in Headwater Streams of the Adirondack Mountains, New York (2017, methodology): “, Brook Trout DNAwas strongly amplified in all IPCs) due to the use of TaqMan Environmental Master Mix, which efficiently “releases” PCR inhibition (Takahara et al. 2015).”
Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples (2018, methodology): “A majority of these studies investigated the presence of exotic fish such as common carp (Cyprinus carpio; Eichmiller et al. 2014; Takahara et al. 2015), silver and bighead carp (Hypophthalmichthys molitrix and nobilis; Jerde et al. 2013; Farrington et al. 2015), African jewelfish (Hemichromis…”
Using eDNA to develop a national citizen science-based monitoring programme for the great crested newt (Triturus cristatus) (2015, background): “Also in this special issue, Takahara et al. (2014) found that less eDNA was extracted from water samples that were frozen, compared to unfrozen samples.”
A simple method for preserving environmental DNA in water samples at ambient temperature by addition of cationic surfactant (2017, methodology): “2016), freezing the water samples (Takahara et al. 2015) and on-ice preservation/ethanol fixation/immersion in lysis buffers of obtained filter samples (Goldberg et al.”